View source for 9bfp


==Cryo-EM structure of Sevenless extracellular domain (monomer)==
<StructureSection load='9bfp' size='340' side='right'caption='[[9bfp]], [[Resolution|resolution]] 2.78&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9bfp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9BFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9BFP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.78&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9bfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9bfp OCA], [https://pdbe.org/9bfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9bfp RCSB], [https://www.ebi.ac.uk/pdbsum/9bfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9bfp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/7LESS_DROME 7LESS_DROME] Receptor for an extracellular signal required to instruct a cell to differentiate into an R7 photoreceptor. The ligand for sev is the boss (bride of sevenless) protein on the surface of the neighboring R8 cell.<ref>PMID:2840202</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sevenless (Sev) is a Drosophila receptor tyrosine kinase (RTK) required for the specification of the R7 photoreceptor. It is cleaved into alpha and beta subunits and binds the ectodomain of the G-protein-coupled receptor bride of sevenless (Boss). Previous work showed that the Boss ectodomain could bind but not activate Sev; rather, the whole seven-pass transmembrane Boss was required. Here, we show that Sev does not need to be cleaved to function and that a single-pass transmembrane form of Boss activates Sev. We use cryo-electron microscopy and biophysical methods to determine the structural basis of ligand binding and pH-dependent dimerization of Sev, and we discuss the implications in the process of Sev activation. The Sev human homolog, receptor oncogene from sarcoma 1 (ROS1), is associated with oncogenic transformations, and we discuss their structural similarities.

Structures and pH-dependent dimerization of the sevenless receptor tyrosine kinase.,Cerutti G, Arias R, Bahna F, Mannepalli S, Katsamba PS, Ahlsen G, Kloss B, Bruni R, Tomlinson A, Shapiro L Mol Cell. 2024 Dec 5;84(23):4677-4690.e6. doi: 10.1016/j.molcel.2024.10.017. Epub , 2024 Nov 6. PMID:39510067<ref>PMID:39510067</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9bfp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Cerutti G]]
[[Category: Shapiro L]]