View source for 9bmk


==GH5_4 endo-beta(1,3/1,4)-glucanase E331A from Segatella copri in complex with cellotriose==
<StructureSection load='9bmk' size='340' side='right'caption='[[9bmk]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9bmk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Segatella_copri_DSM_18205 Segatella copri DSM 18205]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9BMK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9BMK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.21&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PRD_900021:beta-cellotriose'>PRD_900021</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9bmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9bmk OCA], [https://pdbe.org/9bmk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9bmk RCSB], [https://www.ebi.ac.uk/pdbsum/9bmk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9bmk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D1PD14_9BACT D1PD14_9BACT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mixed-linkage beta(1,3)/beta(1,4)-glucan (MLG) is abundant in the human diet through the ingestion of cereal grains and is widely associated with healthful effects on metabolism and cholesterol levels. MLG is also a major source of fermentable glucose for the human gut microbiota (HGM). Bacteria from the family Prevotellaceae are highly represented in the HGM of individuals who eat plant-rich diets, including certain indigenous people and vegetarians in postindustrial societies. Here, we have defined and functionally characterized an exemplar Prevotellaceae MLG polysaccharide utilization locus (MLG-PUL) in the type-strain Segatella copri (syn. Prevotella copri) DSM 18205 through transcriptomic, biochemical, and structural biological approaches. In particular, structure-function analysis of the cell-surface glycan-binding proteins and glycoside hydrolases of the S. copri MLG-PUL revealed the molecular basis for glycan capture and saccharification. Notably, syntenic MLG-PULs from human gut, human oral, and ruminant gut Prevotellaceae are distinguished from their counterparts in Bacteroidaceae by the presence of a beta(1,3)-specific endo-glucanase from glycoside hydrolase family 5, subfamily 4 (GH5_4) that initiates MLG backbone cleavage. The definition of a family of homologous MLG-PULs in individual species enabled a survey of nearly 2000 human fecal microbiomes using these genes as molecular markers, which revealed global population-specific distributions of Bacteroidaceae- and Prevotellaceae-mediated MLG utilization. Altogether, the data presented here provide new insight into the molecular basis of beta-glucan metabolism in the HGM, as a basis for informing the development of approaches to improve the nutrition and health of humans and other animals.

The molecular basis of cereal mixed-linkage beta-glucan utilization by the human gut bacterium Segatella copri.,Golisch B, Cordeiro RL, Fraser ASC, Briggs J, Stewart WA, Van Petegem F, Brumer H J Biol Chem. 2024 Sep;300(9):107625. doi: 10.1016/j.jbc.2024.107625. Epub 2024 , Aug 8. PMID:39122003<ref>PMID:39122003</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9bmk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Segatella copri DSM 18205]]
[[Category: Brumer H]]
[[Category: Cordeiro RL]]
[[Category: Golisch B]]
[[Category: Van Petegem F]]