View source for 9cp8


==Crystal structure of DHPS-3-dehydrogenase, HpsN from Cupriavidus pinatubonensis in complex with product (R-sulfolactate) and NADH==
<StructureSection load='9cp8' size='340' side='right'caption='[[9cp8]], [[Resolution|resolution]] 1.57&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9cp8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_pinatubonensis_JMP134 Cupriavidus pinatubonensis JMP134]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9CP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9CP8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.57&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3SL:(2R)-3-SULFOLACTIC+ACID'>3SL</scene>, <scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9cp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9cp8 OCA], [https://pdbe.org/9cp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9cp8 RCSB], [https://www.ebi.ac.uk/pdbsum/9cp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9cp8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HPSN_CUPPJ HPSN_CUPPJ] Catalyzes the NAD-dependent oxidation of (R)-2,3-dihydroxypropane-1-sulfonate to (R)-3-sulfolactate.<ref>PMID:20150239</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
2,3-Dihydroxypropanesulfonate (DHPS) and sulfolactate (SL) are environmentally important organosulfur compounds that play key roles as metabolic currencies in the sulfur cycle. Despite their prevalence, the pathways governing DHPS and SL production remain poorly understood. Here, we study DHPS-3-dehydrogenase from Cupriavidus pinatubonensis (CpHpsN), a bacterium capable of utilizing DHPS as a sole carbon source. Kinetic analysis of CpHpsN reveals a strict preference for R-DHPS, catalyzing its 4-electron oxidation to R-SL, with high specificity for NAD(+) over NADP(+). The 3D structure of CpHpsN in complex with Zn(2+), NADH and R-SL, elucidated through X-ray crystallography, reveals a fold akin to bacterial and plant histidinol dehydrogenases with similar coordination geometry around the octahedral Zn(2+) centre and involving the sulfonate group as a ligand. A key residue, His126, distinguishes DHPS dehydrogenases from histidinol dehydrogenases, by structural recognition of the sulfonate substrate of the former. Site-directed mutagenesis pinpoints Glu318, His319, and Asp352 as active-site residues important for the catalytic activity of CpHpsN. Taxonomic and pathway distribution analysis reveals the prevalence of HpsN homologues within different pathways of DHPS catabolism and across bacterial classes including Alpha-, Beta-, Gamma-, and Deltaproteobacteria and Desulfobacteria, emphasizing its importance in the biogeochemical sulfur cycle.

Structural and kinetic insights into the stereospecific oxidation of R-2,3-dihydroxypropanesulfonate by DHPS-3-dehydrogenase from Cupriavidus pinatubonensis.,Burchill L, Kaur A, Nastasovici A, Lee M, Williams SJ Chem Sci. 2024 Sep 9. doi: 10.1039/d4sc05114a. PMID:39263660<ref>PMID:39263660</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9cp8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Cupriavidus pinatubonensis JMP134]]
[[Category: Large Structures]]
[[Category: Lee M]]