View source for 9cr5


==Crystal structure of GNAT superfamily acetyltransferase PA2271 from Pseudomonas aeruginosa==
<StructureSection load='9cr5' size='340' side='right'caption='[[9cr5]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9cr5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9CR5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9CR5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9cr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9cr5 OCA], [https://pdbe.org/9cr5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9cr5 RCSB], [https://www.ebi.ac.uk/pdbsum/9cr5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9cr5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ATSE1_PSEAE ATSE1_PSEAE] Catalyzes the transfer of an acetyl group from acetyl coenzyme A (AcCoA) to an acceptor substrate and releases both CoA and the acetylated product. It can use a variety of substrates including spermidine, spermine and N(8)-acetylspermidine, 7-aminocephalosporanic acid, colistin and thiamine.<ref>PMID:23184347</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Polyamines within the cell are tightly regulated by spermidine/spermine N-acetyltransferase (SSAT) enzymes. While several SSATs have been investigated in different bacterial species, there is still a significant gap in knowledge about which proteins are functional SSATs in many organisms. For example, while it is known that Pseudomonas aeruginosa synthesizes the polyamine spermidine, the SSAT that acetylates this molecule and its importance in regulating intracellular polyamines remains unknown. We previously identified a candidate Gcn5-related N-acetyltransferase (GNAT) protein from P. aeruginosa (PA2271) that could fulfill this role since it acetylates spermidine, but no further studies were conducted. Here, we explored the structure/function relationship of the PA2271 protein by determining its X-ray crystal structure and performing enzyme kinetics assays. We also identified active site residues that are essential for catalysis and substrate binding. As the study progressed, we encountered results that led us to explore the importance of four cysteine residues on enzyme activity and disulfide bond formation or modification of cysteine residues. We found these cysteine residues in PA2271 are important for protein solubility and activity, and there is an interrelationship between cysteine residues that contribute to these effects. Furthermore, we also found disulfide bonds could form between C(121) and C(165) and speculate that these residues may contribute to redox regulation of PA2271 protein activity.

Structural, functional, and regulatory evaluation of a cysteine post-translationally modified Gcn5-related N-acetyltransferase.,Uychoco P, Majorek KA, Ives AN, Le VTB, Caro De Silva PL, Paurus VL, Attah IK, Lipton MS, Minor W, Kuhn ML Biochem Biophys Res Commun. 2025 Feb 8;748:151299. doi: , 10.1016/j.bbrc.2025.151299. Epub 2025 Jan 11. PMID:39826527<ref>PMID:39826527</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9cr5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Cooper DR]]
[[Category: Joachimiak A]]
[[Category: Majorek KA]]
[[Category: Minor W]]