View source for 9cyt


==Cryo-EM structure of MRV outer shell==
<StructureSection load='9cyt' size='340' side='right'caption='[[9cyt]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9cyt]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mammalian_orthoreovirus_3_Dearing Mammalian orthoreovirus 3 Dearing]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9CYT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9CYT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9cyt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9cyt OCA], [https://pdbe.org/9cyt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9cyt RCSB], [https://www.ebi.ac.uk/pdbsum/9cyt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9cyt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LMBD2_REOVD LMBD2_REOVD] Outer capsid protein involved in mRNA capping. Catalyzes the last 3 enzymatic activities for formation of the 5' cap structure on the viral plus-strand transcripts, namely the RNA guanylyltransferase, RNA-7N- and RNA-2'O-methyltransferase activities.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Virus-induced cellular condensates, or viral factories, are poorly understood high-density phases where replication of many viruses occurs. Here, by cryogenic electron tomography (cryoET) of focused ion beam (FIB) milling-produced lamellae of mammalian reovirus (MRV)-infected cells, we visualized the molecular organization and interplay (i.e., "molecular sociology") of host and virus in 3D at two time points post-infection, enabling a detailed description of these condensates and a mechanistic understanding of MRV replication within them. Expanding over time, the condensate fashions host ribosomes at its periphery, and host microtubules, lipid membranes, and viral molecules in its interior, forming a 3D architecture that supports the dynamic processes of viral genome replication and capsid assembly. A total of six MRV assembly intermediates are identified inside the condensate: star core, empty and genome-containing cores, empty and full virions, and outer shell particle. Except for star core, these intermediates are visualized at atomic resolution by cryogenic electron microscopy (cryoEM) of cellular extracts. The temporal sequence and spatial rearrangement among these viral intermediates choreograph the viral life cycle within the condensates. Together, the molecular sociology of MRV-induced cellular condensate highlights the functional advantage of transient enrichment of molecules at the right location and time for viral replication.

Molecular sociology of virus-induced cellular condensates supporting reovirus assembly and replication.,Liu X, Xia X, Martynowycz MW, Gonen T, Zhou ZH Nat Commun. 2024 Dec 6;15(1):10638. doi: 10.1038/s41467-024-54968-7. PMID:39639006<ref>PMID:39639006</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 9cyt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mammalian orthoreovirus 3 Dearing]]
[[Category: Gonen T]]
[[Category: Liu XY]]
[[Category: Martynowycz MW]]
[[Category: Xia X]]
[[Category: Zhou ZH]]