View source for 9d2h


==Stigmatella aurantica bacteriophytochrome protein 2 (SaBphP2), photosensory core module, investigated at ESRF(EBS) ID29. Dark Structure.==
<StructureSection load='9d2h' size='340' side='right'caption='[[9d2h]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9d2h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Stigmatella_aurantiaca Stigmatella aurantiaca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9D2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9D2H FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9d2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9d2h OCA], [https://pdbe.org/9d2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9d2h RCSB], [https://www.ebi.ac.uk/pdbsum/9d2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9d2h ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q09E27_STIAD Q09E27_STIAD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The upgrade of the European Synchrotron Radiation Facility (ESRF) in Grenoble, France to an Extremely Brilliant Source (EBS) is expected to enable time-resolved synchrotron serial crystallography (SSX) experiments with sub-millisecond time resolution. ID29 is a new beamline dedicated to SSX experiments at ESRF-EBS. Here, we report experiments emerging from the initial phase of user operation at ID29. We first used microcrystals of photoactive yellow protein as a model system to exploit the potential of microsecond pulses for SSX. Subsequently, we investigated microcrystals of cytochrome c nitrite reductase (ccNiR) with microsecond X-ray pulses. CcNiR is a decaheme protein that is ideal for the investigation of radiation damage at the various heme-iron sites. Finally, we performed a proof-of-concept subsecond time-resolved SSX experiment by photoactivating microcrystals of a myxobacterial phytochrome.

Exploiting fourth-generation synchrotron radiation for enzyme and photoreceptor characterization.,Malla TN, Muniyappan S, Menendez D, Ogukwe F, Dale AN, Clayton JD, Weatherall DD, Karki P, Dangi S, Mandella V, Pacheco AA, Stojkovic EA, Rose SL, Orlans J, Basu S, de Sanctis D, Schmidt M IUCrJ. 2025 Jan 1;12(Pt 1):36-48. doi: 10.1107/S2052252524010868. PMID:39575537<ref>PMID:39575537</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9d2h" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Stigmatella aurantiaca]]
[[Category: Malla TN]]
[[Category: Schmidt M]]