View source for 9die


==Structure of phospholipase D BetaIB1i from Sicarius terrosus venom, H47N mutant bound to product and substrate sphingolipids at 1.85 A resolution==
<StructureSection load='9die' size='340' side='right'caption='[[9die]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9die]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sicarius_terrosus Sicarius terrosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9DIE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9DIE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1A43:2-aminoethyl+(2S,3R,4E)-2-dodecanamido-3-hydroxyheptadec-4-en-1-yl+hydrogen+(S)-phosphate'>A1A43</scene>, <scene name='pdbligand=A1A44:~{N}-[(4~{R},5~{S})-2-oxidanyl-2-oxidanylidene-4-[(~{E})-tetradec-1-enyl]-1,3,2$l^{5}-dioxaphosphinan-5-yl]dodecanamide'>A1A44</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9die FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9die OCA], [https://pdbe.org/9die PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9die RCSB], [https://www.ebi.ac.uk/pdbsum/9die PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9die ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BIB11_SICTE BIB11_SICTE] Dermonecrotic toxins cleave the phosphodiester linkage between the phosphate and headgroup of certain phospholipids (sphingolipid and lysolipid substrates), forming an alcohol (often choline) and a cyclic phosphate (PubMed:25752604). This toxin acts on lysophosphatidylethanolamine (LPE) and ceramide phosphoethanolamine (CPE) with high activity (PubMed:25752604). This toxin acts on sphingomyelin (SM) with very low activity and is not active on lysophosphatidylserine (LPS), lysophosphatidylcholine (LPC) and lysophosphatidylglycerol (LPG) (PubMed:25752604). It acts by transphosphatidylation, releasing exclusively cyclic phosphate as second products (PubMed:25752604). It is not surprising that spider toxins have affinity for ethanolamine-containing sphingolipids since they are common in insect prey (PubMed:25752604). Induces dermonecrosis, hemolysis, increased vascular permeability, edema, inflammatory response, and platelet aggregation (By similarity).[UniProtKB:P0CE80]<ref>PMID:25752604</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sicarius terrosus]]
[[Category: Binford GJ]]
[[Category: Cordes MH]]
[[Category: Montfort WR]]
[[Category: Sundman AK]]