View source for 9dx4


==EcRuvB T102R mutant==
<StructureSection load='9dx4' size='340' side='right'caption='[[9dx4]], [[Resolution|resolution]] 3.31&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9dx4]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9DX4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9DX4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.31&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9dx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9dx4 OCA], [https://pdbe.org/9dx4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9dx4 RCSB], [https://www.ebi.ac.uk/pdbsum/9dx4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9dx4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RUVB_ECOLI RUVB_ECOLI] The RuvABC complex is involved in recombinational repair of UV or chemically damaged DNA (PubMed:6374379). The complex also plays an important role in the rescue of blocked DNA replication forks via replication fork reversal (RFR); RFR and homologous recombination required for UV light survival can be separated (PubMed:16424908, PubMed:18942176, PubMed:9814711). This subunit has a weak ATPase activity that is inhibited by its ADP product; binds ADP better than ATP (PubMed:2529252). Promotes Holliday junction (HJ) branch migration in conjunction with RuvA. Binds to HJ cruciform DNA; in the presence of RuvA, ATP and Mg(2+) the junction is dissociated. Hydrolyzable (d)NTPs can replace ATP but other analogs cannot (PubMed:1608954, PubMed:1617728, PubMed:6374379, PubMed:8393934). The RuvB hexamer acts as a pump, pulling DNA into and through the RuvAB complex (PubMed:9078376). Can bypass UV-induced lesions (PubMed:1617728) and physically cross-linked DNA strands (PubMed:10662672), suggesting RuvB does not unwind large sections of DNA. RuvA gives specificity by binding to cruciform junctions, while the RuvB ATPase provides the motor force for branch migration; excess RuvB can promote branch migration in the absence of RuvA (PubMed:10662672, PubMed:1617728). In vitro the RuvA-RuvB complex has 5'-3' helicase activity that is ATP-dependent and works best on short dsDNA hybrids; 52 and 66-nucleotide (nt) pairs are easily displaced, hybrids greater than 140-nts are not (PubMed:8433990). RuvA stimulates the weak ATPase activity of RuvB in the presence of DNA; HJ DNA stimulates ATPase about 10-fold (PubMed:1435721, PubMed:1833759, PubMed:8393934).[HAMAP-Rule:MF_00016]<ref>PMID:10662672</ref> <ref>PMID:1435721</ref> <ref>PMID:1608954</ref> <ref>PMID:1617728</ref> <ref>PMID:16424908</ref> <ref>PMID:1833759</ref> <ref>PMID:18942176</ref> <ref>PMID:2529252</ref> <ref>PMID:6374379</ref> <ref>PMID:8393934</ref> <ref>PMID:8433990</ref> <ref>PMID:9078376</ref> <ref>PMID:9814711</ref>   An in vitro resolvase system that forms and processes HJ has been reconstituted with DNA substrates, RuvA, RuvB and RuvC. RuvA-RuvB increases the rate of strand exchange (branch migration), dissociates the RecA filament and allows RuvC to cleave in both orientations at the cruciform junction (PubMed:10421637, PubMed:9160752). HJ-RuvA-RuvB-RuvC complexes resolve Holliday junctions and also undergo branch migration, providing evidence for a coupled branch migration/HJ resolution reaction (PubMed:10421637).<ref>PMID:10421637</ref> <ref>PMID:9160752</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Fu TM]]
[[Category: Rish AD]]