View source for 9dx6


==Crystal structure of Plasmodium vivax (Palo Alto) PvAMA1 in complex with human Fab 826827==
<StructureSection load='9dx6' size='340' side='right'caption='[[9dx6]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9dx6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Plasmodium_vivax Plasmodium vivax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9DX6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9DX6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9dx6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9dx6 OCA], [https://pdbe.org/9dx6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9dx6 RCSB], [https://www.ebi.ac.uk/pdbsum/9dx6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9dx6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B9TXF7_PLAVI B9TXF7_PLAVI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
New therapeutics are necessary for preventing Plasmodium vivax malaria due to easy transmissibility and dormancy in the liver that increases the clinical burden due to recurrent relapse. In this manuscript we characterize 12 Pv Apical Membrane Antigen 1 (PvAMA1) specific human monoclonal antibodies from Peripheral Blood Mononuclear Cells of a Pv-exposed individual. PvAMA1 is essential for sporozoite and merozoite invasion, making it a unique therapeutic target. We show that humAb 826827 blocks the invasion of human reticulocytes using Pv clinical isolates and inhibits sporozoite invasion of human hepatocytes in vitro (IC(50) of 0.3 - 3.7 microg/mL). Inoculation of human liver transgenic (FRG-humHep) female mice with humAb 826827 significantly reduces liver infection in vivo. The crystal structure of rPvAMA1 bound to 826827 shows that 826827 partially occupies the highly conserved hydrophobic groove in PvAMA1 that binds its known receptor, RON2. We have isolated a potent humAb that is isolate-transcendent, blocks both pre-erythrocytic and blood stage infection, and could be a potential therapy for Pv.

Potent AMA1-specific human monoclonal antibody against Plasmodium vivax Pre-erythrocytic and Blood Stages.,Winnicki AC, Dietrich MH, Yeoh LM, Carias LL, Roobsoong W, Drago CL, Malachin AN, Redinger KR, Feufack-Donfack LB, Baldor L, Jung NC, McLaine OS, Skomorovska-Prokvolit Y, Orban A, Opi DH, Kirtley P, Nielson K, Aleshnick M, Zanghi G, Rezakhani N, Vaughan AM, Wilder BK, Sattabongkot J, Tham WH, Popovici J, Beeson JG, Bosch J, King CL Nat Commun. 2024 Dec 4;15(1):10556. doi: 10.1038/s41467-024-53848-4. PMID:39632799<ref>PMID:39632799</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9dx6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Plasmodium vivax]]
[[Category: Dietrich MH]]
[[Category: Jung NC]]
[[Category: Tham WH]]