View source for 9ep1


==Structure of the Integrator arm module containing INTS10/13/14/15 subunits (state 2)==
<StructureSection load='9ep1' size='340' side='right'caption='[[9ep1]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9ep1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9EP1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ep1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ep1 OCA], [https://pdbe.org/9ep1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ep1 RCSB], [https://www.ebi.ac.uk/pdbsum/9ep1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ep1 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Integrator is a multi-subunit protein complex responsible for premature transcription termination of coding and non-coding RNAs. This is achieved via two enzymatic activities, RNA endonuclease and protein phosphatase, acting on the promoter-proximally paused RNA polymerase â¡ (RNAPâ¡). Yet, it remains unclear how Integrator assembly and recruitment are regulated and what the functions of many of its core subunits are. Here, we report the structures of two human Integrator sub-complexes: INTS10/13/14/15 and INTS5/8/10/15, and an integrative model of the fully assembled Integrator bound to the RNAPâ¡ paused elongating complex (PEC). An in silico protein-protein interaction screen of over 1,500 human transcription factors (TFs) identified ZNF655 as a direct interacting partner of INTS13 within the fully assembled Integrator. We propose a model wherein INTS13 acts as a platform for the recruitment of TFs that could modulate the stability of the Integrator's association at specific loci and regulate transcription attenuation of the target genes.

Structural basis of the Integrator complex assembly and association with transcription factors.,Razew M, Fraudeau A, Pfleiderer MM, Linares R, Galej WP Mol Cell. 2024 May 27:S1097-2765(24)00431-3. doi: 10.1016/j.molcel.2024.05.009. PMID:38823386<ref>PMID:38823386</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 9ep1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Galej WP]]
[[Category: Razew M]]