View source for 9epn


==Crystal structure of HprS histidine kinase cytoplasmic fragment from Escherichia coli==
<StructureSection load='9epn' size='340' side='right'caption='[[9epn]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9epn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EPN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9EPN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GCH:GLYCOCHOLIC+ACID'>GCH</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9epn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9epn OCA], [https://pdbe.org/9epn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9epn RCSB], [https://www.ebi.ac.uk/pdbsum/9epn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9epn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HPRS_ECOLI HPRS_ECOLI] Member of a two-component regulatory system HprR/HprS involved in response to hydrogen peroxide (PubMed:25568260, PubMed:27983483). Senses H(2)O(2), maybe via the redox state of the membrane (PubMed:27983483). Activates HprR by phosphorylation (PubMed:15522865). Can also phosphorylate CusR (PubMed:15522865).<ref>PMID:15522865</ref> <ref>PMID:25568260</ref> <ref>PMID:27983483</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The HprSR constitutes the bacterial two-component regulatory system engaged by Escherichia coli to reduce the damaging effects of reactive chlorine and oxygen species present in its cytosol. Hypochlorous acid (HOCl) has been shown to be the molecule capable of activating of the HprSR system. HOCl is produced upon pathogen invasion by phagocytic cells of the human innate immune system, particularly neutrophils, to take advantage of its powerful antimicrobial attributes. Therefore, comprehensive studies concerning bacterial sensing and regulatory HprSR system are indispensable in understanding and effectively eliminating pathogens. Here we present the first crystal structure, solved at 1.7 A resolution, of the HprS cytoplasmic domains arranged as a homodimer. In both protomers, the catalytic ATP-binding domain contains a non-hydrolysable ATP analog coordinated by a magnesium ion. This structure allowed us to provide a detailed characterization of kinase-substrate interaction. Furthermore, the structural data are supported by biophysical studies of kinase interaction with cognate response regulator HprR and substrate ATP. The kinase activity is also assessed in the presence or absence of HprR.

Structural and biophysical characterization of the cytoplasmic domains of HprS kinase and its interactions with the cognate regulator HprR.,Koczurowska A, Carrillo DR, Alai MG, Zaklos-Szyda M, Bujacz G, Pietrzyk-Brzezinska AJ Arch Biochem Biophys. 2025 Feb;764:110269. doi: 10.1016/j.abb.2024.110269. Epub , 2024 Dec 15. PMID:39681306<ref>PMID:39681306</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9epn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Bujacz G]]
[[Category: Koczurowska A]]
[[Category: Pietrzyk-Brzezinska AJ]]