View source for 9esa


==Aurora-C with SER mutation in complex with INCENP peptide==
<StructureSection load='9esa' size='340' side='right'caption='[[9esa]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9esa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9ESA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9ESA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9esa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9esa OCA], [https://pdbe.org/9esa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9esa RCSB], [https://www.ebi.ac.uk/pdbsum/9esa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9esa ProSAT]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/AURKC_HUMAN AURKC_HUMAN] Male infertility due to large-headed multiflagellar polyploid spermatozoa. The disease is caused by mutations affecting the gene represented in this entry.
== Function ==
[https://www.uniprot.org/uniprot/AURKC_HUMAN AURKC_HUMAN] Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Plays also a role in meiosis and more particularly in spermatogenesis. Has redundant cellular functions with AURKB and can rescue an AURKB knockdown. Like AURKB, AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28'. AURKC phosphorylates the CPC complex subunits BIRC5/survivin and INCENP leading to increased AURKC activity. Phosphorylates TACC1, another protein involved in cell division, at 'Ser-228'.<ref>PMID:15316025</ref> <ref>PMID:15499654</ref> <ref>PMID:15670791</ref> <ref>PMID:15938719</ref> <ref>PMID:21493633</ref> <ref>PMID:21531210</ref> <ref>PMID:27332895</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A key prerequisite for the successful application of protein crystallography in drug discovery is to establish a robust crystallization system for a new drug-target protein fast enough to deliver crystal structures when the first inhibitors have been identified in the hit-finding campaign or, at the latest, in the subsequent hit-to-lead process. The first crucial step towards generating well folded proteins with a high likelihood of crystallizing is the identification of suitable truncation variants of the target protein. In some cases an optimal length variant alone is not sufficient to support crystallization and additional surface mutations need to be introduced to obtain suitable crystals. In this contribution, four case studies are presented in which rationally designed surface modifications were key to establishing crystallization conditions for the target proteins (the protein kinases Aurora-C, IRAK4 and BUB1, and the KRAS-SOS1 complex). The design process which led to well diffracting crystals is described and the crystal packing is analysed to understand retrospectively how the specific surface mutations promoted successful crystallization. The presented design approaches are routinely used in our team to support the establishment of robust crystallization systems which enable structure-guided inhibitor optimization for hit-to-lead and lead-optimization projects in pharmaceutical research.

Surface-mutagenesis strategies to enable structural biology crystallization platforms.,Schaefer M, Putter V, Hilpmann A, Egner U, Holton SJ, Hillig RC Acta Crystallogr D Struct Biol. 2024 Sep 1. doi: 10.1107/S2059798324007939. PMID:39207897<ref>PMID:39207897</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 9esa" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Hillig RC]]