View source for 9ex6


==CryoEM structure of sTeLIC nanodisc in closed state==
<StructureSection load='9ex6' size='340' side='right'caption='[[9ex6]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9ex6]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Endosymbiont_of_Tevnia_jerichonana_(vent_Tica) Endosymbiont of Tevnia jerichonana (vent Tica)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EX6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9EX6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.35&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C14:TETRADECANE'>C14</scene>, <scene name='pdbligand=D10:DECANE'>D10</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=HEX:HEXANE'>HEX</scene>, <scene name='pdbligand=OCT:N-OCTANE'>OCT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ex6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ex6 OCA], [https://pdbe.org/9ex6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ex6 RCSB], [https://www.ebi.ac.uk/pdbsum/9ex6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ex6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G2FID1_9GAMM G2FID1_9GAMM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND AND PURPOSE: Allosteric modulation of pentameric ligand-gated ion channels (pLGICs) are critical for the action of neurotransmitters and many psychoactive drugs. However, details of their modulatory mechanisms remain unclear, especially beyond the orthosteric neurotransmitter-binding sites. The recently reported prokaryotic symbiont of Tevnia jerichonana ligand-gated ion channel (sTeLIC), a pH-gated homologue of eukaryotic receptors in the pLGIC family, is thought to be modulated by aromatic compounds via a relatively uncharacterised modulatory site in the extracellular vestibule. EXPERIMENTAL APPROACH: We have characterised the effects of psychostimulant derivatives on sTeLIC using two-electrode voltage-clamp electrophysiology in the presence and absence of engineered mutations, and determined X-ray and cryo-EM structures of the channel in both closed and open states. KEY RESULTS: We have shown that sTeLIC is sensitive to potentiation by several amphiphilic compounds, which preferentially bind to a vestibular pocket in the contracted open-state extracellular domain. CONCLUSIONS AND IMPLICATIONS: This work provides a detailed structure-function mechanism for allosteric potentiation via a noncanonical ligand site, with potential conservation of the eukaryotic pentameric ligand-gated ion channels.

Vestibular modulation by stimulant derivatives in a pentameric ligand-gated ion channel.,Karlsson E, Anden O, Fan C, Fourati Z, Haouz A, Zhuang Y, Howard RJ, Delarue M, Lindahl E Br J Pharmacol. 2025 Mar 11. doi: 10.1111/bph.70011. PMID:40065647<ref>PMID:40065647</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9ex6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Fan C]]
[[Category: Howard RJ]]
[[Category: Lindahl E]]