View source for 9f9g


==Laser excitation effects on BR: Reprocessed Dark from Nogly et al.==
<StructureSection load='9f9g' size='340' side='right'caption='[[9f9g]], [[Resolution|resolution]] 1.44&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9f9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9F9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9F9G FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.44&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LI1:1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL'>LI1</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9f9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9f9g OCA], [https://pdbe.org/9f9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9f9g RCSB], [https://www.ebi.ac.uk/pdbsum/9f9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9f9g ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA] Light-driven proton pump.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Time-resolved serial crystallography at X-ray Free Electron Lasers offers the opportunity to observe ultrafast photochemical reactions at the atomic level. The technique has yielded exciting molecular insights into various biological processes including light sensing and photochemical energy conversion. However, to achieve sufficient levels of activation within an optically dense crystal, high laser power densities are often used, which has led to an ongoing debate to which extent photodamage may compromise interpretation of the results. Here we compare time-resolved serial crystallographic data of the bacteriorhodopsin K-intermediate collected at laser power densities ranging from 0.04 to 2493 GW/cm(2) and follow energy dissipation of the absorbed photons logarithmically from picoseconds to milliseconds. Although the effects of high laser power densities on the overall structure are small, in the upper excitation range we observe significant changes in retinal conformation and increased heating of the functionally critical counterion cluster. We compare light-activation within crystals to that in solution and discuss the impact of the observed changes on bacteriorhodopsin biology.

Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate.,Bertrand Q, Nogly P, Nango E, Kekilli D, Khusainov G, Furrer A, James D, Dworkowski F, Skopintsev P, Mous S, Martiel I, Borjesson P, Ortolani G, Huang CY, Kepa M, Ozerov D, Brunle S, Panneels V, Tanaka T, Tanaka R, Tono K, Owada S, Johnson PJM, Nass K, Knopp G, Cirelli C, Milne C, Schertler G, Iwata S, Neutze R, Weinert T, Standfuss J Nat Commun. 2024 Nov 27;15(1):10278. doi: 10.1038/s41467-024-54422-8. PMID:39604356<ref>PMID:39604356</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9f9g" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Halobacterium salinarum]]
[[Category: Large Structures]]
[[Category: Bertrand Q]]
[[Category: Standfuss J]]
[[Category: Weinert T]]