View source for 9fmh


==PsiM N247M in complex with SAH and norbaeocystin==
<StructureSection load='9fmh' size='340' side='right'caption='[[9fmh]], [[Resolution|resolution]] 0.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9fmh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Psilocybe_cubensis Psilocybe cubensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9FMH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9FMH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=XP6:[3-(2-azanylethyl)-1~{H}-indol-4-yl]+dihydrogen+phosphate'>XP6</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9fmh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9fmh OCA], [https://pdbe.org/9fmh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9fmh RCSB], [https://www.ebi.ac.uk/pdbsum/9fmh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9fmh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PSIM_PSICU PSIM_PSICU] N-methyltransferase; part of the gene cluster that mediates the biosynthesis of psilocybin, a psychotropic tryptamine-derived natural product (PubMed:28763571, PubMed:31150155). The first step in the pathway is the decarboxylation of L-tryptophan to tryptamine by the decarboxylase psiD (PubMed:28763571, PubMed:31150155). 4-hydroxy-L-tryptophan is accepted as substrate by psiD as well (PubMed:28763571). The cytochrome P450 monooxygenase psiH then converts tryptamine to 4-hydroxytryptamine (PubMed:28763571). The kinase psiK catalyzes the 4-O-phosphorylation step by converting 4-hydroxytryptamine into norbaeocystin (PubMed:28763571, PubMed:31150155). The methyltransferase psiM then catalyzes iterative methyl transfer to the amino group of norbaeocystin to yield psilocybin via a monomethylated intermediate, baeocystin (PubMed:28763571, PubMed:31150155). 4-hydroxy-6-methyl-l-tryptophancan also be converted the decarboxylase PsiD, kinase PsiK, and methyltransferase PsiM into respectively 6-methyl-norbaeocystin, 6-methylbaeocystin, and 6-methylpsilocybin (PubMed:31150155).<ref>PMID:28763571</ref> <ref>PMID:31150155</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Psilocybe cubensis]]
[[Category: Hudspeth J]]
[[Category: Rupp B]]
[[Category: Werten S]]