View source for 9fwv


==Rubisco in native beta-carboxysomes==
<StructureSection load='9fwv' size='340' side='right'caption='[[9fwv]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9fwv]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9FWV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9FWV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9fwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9fwv OCA], [https://pdbe.org/9fwv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9fwv RCSB], [https://www.ebi.ac.uk/pdbsum/9fwv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9fwv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CCMM_SYNE7 CCMM_SYNE7] The presence of two potential DNA-binding regions suggests this protein may be a transcriptional regulator.
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== Publication Abstract from PubMed ==
Carboxysomes are anabolic bacterial microcompartments that play an essential role in CO2 fixation in cyanobacteria. This self-assembling proteinaceous organelle uses a polyhedral shell constructed by hundreds of shell protein paralogs to encapsulate the key CO2-fixing enzymes Rubisco and carbonic anhydrase. Deciphering the precise arrangement and structural organization of Rubisco enzymes within carboxysomes is crucial for understanding carboxysome formation and overall functionality. Here, we employed cryoelectron tomography and subtomogram averaging to delineate the 3D packaging of Rubiscos within beta-carboxysomes in the freshwater cyanobacterium Synechococcus elongatus PCC7942 grown under low light. Our results revealed that Rubiscos are arranged in multiple concentric layers parallel to the shell within the beta-carboxysome lumen. We also detected Rubisco binding with the scaffolding protein CcmM in beta-carboxysomes, which is instrumental for Rubisco encapsulation and beta-carboxysome assembly. Using Quantification conCATamer-based quantitative MS, we determined the absolute stoichiometric composition of the entire beta-carboxysome. This study provides insights into the assembly principles and structural variation of beta-carboxysomes, which will aid in the rational design and repurposing of carboxysome nanostructures for diverse bioengineering applications.

Rubisco packaging and stoichiometric composition of the native beta-carboxysome in Synechococcus elongatus PCC7942.,Sun Y, Sheng Y, Ni T, Ge X, Sarsby J, Brownridge PJ, Li K, Hardenbrook N, Dykes GF, Rockliffe N, Eyers CE, Zhang P, Liu LN Plant Physiol. 2024 Dec 24;197(1):kiae665. doi: 10.1093/plphys/kiae665. PMID:39680612<ref>PMID:39680612</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9fwv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Synechococcus elongatus PCC 7942 = FACHB-805]]
[[Category: Hardenbrook N]]
[[Category: Li K]]
[[Category: Sheng Y]]