View source for 9gmz


==CryoEM structure of PmcTnsC-dsDNA-AMPPNP in complex with PmcTnsAB hook==
<StructureSection load='9gmz' size='340' side='right'caption='[[9gmz]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9gmz]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Peltigera_membranacea Peltigera membranacea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GMZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9GMZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9gmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gmz OCA], [https://pdbe.org/9gmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9gmz RCSB], [https://www.ebi.ac.uk/pdbsum/9gmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9gmz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A235IFM2_9NOSO A0A235IFM2_9NOSO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CRISPR-associated transposon (CAST) systems employ CRISPR-Cas systems as RNA-directed targeting modules for site-specific transposon DNA insertion. Among them, type I CASTs rely on the coordinated action of the guide RNA-bound Cascade complex and the transposon proteins TniQ, TnsC, and TnsAB. The interaction between the transposase TnsAB and the ATPase TnsC is crucial for transposition activity, yet the underlying molecular details have remained elusive. Here, we investigate the type I-B CAST system from Peltigera membranacea cyanobiont. Cryo-electron microscopic structures of TnsC and its complex with the C-terminal region of TnsAB reveal that TnsC forms a heptameric ring that recruits TnsAB by interacting with its C-terminal tail. In vitro binding assays indicate that TnsAB exclusively interacts with the TnsC heptamer without inducing its disassembly, in contrast to type V-K CAST systems. Mutational analysis of key structural features corroborates the significance of TnsC multimerization and TnsB interaction for transposon activity in vivo. Altogether, these findings offer detailed structural and functional insights into the molecular mechanism of type I-B CAST, with the aim of facilitating their development as genome engineering tools.

Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons.,Finocchio G, Querques I, Chanez C, Speichert KJ, Jinek M Nucleic Acids Res. 2025 Feb 27;53(5):gkaf149. doi: 10.1093/nar/gkaf149. PMID:40103227<ref>PMID:40103227</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9gmz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Peltigera membranacea]]
[[Category: Chanez C]]
[[Category: Finocchio G]]
[[Category: Jinek M]]
[[Category: Querques I]]
[[Category: Speichert KJ]]