View source for 9gpq


==The FK1 domain of FKBP51 in complex with the macrocyclic SAFit analog 12h/p==
<StructureSection load='9gpq' size='340' side='right'caption='[[9gpq]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9gpq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9GPQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9gpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gpq OCA], [https://pdbe.org/9gpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9gpq RCSB], [https://www.ebi.ac.uk/pdbsum/9gpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9gpq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FKBP5_HUMAN FKBP5_HUMAN] Interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Macrocycles are abundantly used by nature to enable cell-permeable bioactive molecules. Synthetic non-peptidic macrocycles are also increasingly considered as modalities for difficult-to-bind proteins but guidelines for macrocyclization are only beginning to emerge. Macrocycles are thought to constrain the available conformations but also to allow for residual flexibility, the latter being poorly understood. Here we show that even medium-sized macrocycles display an unexpected high conformational plasticity, even when bound to their protein target. Minor modification of the linker region of macrocycles can shift the conformational ensemble to distinct conformational subclasses, each constituting distinct three-dimensional scaffolds for further optimization. This led to several new ligands with improved affinity and beneficial physicochemical parameters for the FK506-binding protein 51, a promising target for depression, obesity and chronic pain. Importantly, none of the beneficial modifications could have been identified by classical medicinal chemistry as they only work in the macrocyclic context. Our results show that macrocyclization can do more than keeping loose ends together but rather provide a platform for multiple series of macrocycles with distinct binding modes.

Conformational Plasticity and Binding Affinity Enhancement Controlled by Linker Derivatization in Macrocycles.,Buffa V, Walz C, Meyners C, Zheng M, Sugiarto WO, Repity M, Achaq H, Cica M, Brudy C, Spiske M, Hausch F Angew Chem Int Ed Engl. 2025 Jan 3:e202418512. doi: 10.1002/anie.202418512. PMID:39749563<ref>PMID:39749563</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9gpq" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Buffa V]]
[[Category: Hausch F]]
[[Category: Meyners C]]
[[Category: Walz C]]