View source for 9gr4


==The MKA-RSL - sulfonato-calix[4]arene complex==
<StructureSection load='9gr4' size='340' side='right'caption='[[9gr4]], [[Resolution|resolution]] 1.08&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9gr4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Ralstonia_solanacearum Ralstonia solanacearum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GR4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9GR4 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.08&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDF:BETA-D-FRUCTOPYRANOSE'>BDF</scene>, <scene name='pdbligand=T3Y:25,26,27,28-TETRAHYDROXYPENTACYCLO[19.3.1.1~3,7~.1~9,13~.1~15,19~]OCTACOSA-1(25),3(28),4,6,9(27),10,12,15(26),16,18,21,23-DODECAENE-5,11,17,23-TETRASULFONIC+ACID'>T3Y</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9gr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gr4 OCA], [https://pdbe.org/9gr4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9gr4 RCSB], [https://www.ebi.ac.uk/pdbsum/9gr4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9gr4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0S4TLR1_RALSL A0A0S4TLR1_RALSL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We describe the capture and structuring of disordered N-terminal regions by the macrocycle sulfonato-calix[4]arene (sclx(4)). Using the trimeric beta-propeller Ralstonia solanacearum lectin (RSL) as a scaffold, we generated a series of mutants with extended and dynamic N-termini. Three of the mutants feature an N-terminal methionine-lysine motif. The fourth mutant contains the disordered 8-residue N-terminus of Histone 3, a component of the nucleosome. X-ray crystallography and NMR spectroscopy provide evidence for sclx(4) binding to the flexible N-terminal regions. Three crystal structures reveal that the calixarene recognizes the N-terminal Met-Lys motif, capturing either residue. We provide crystallographic proof for sclx(4) encapsulation of N-terminal methionine. Calixarene capture of intrinsically disordered regions may have applications in regulating protein secondary (and tertiary) structure.

N-Terminal Protein Binding and Disorder-to-Order Transition by a Synthetic Receptor.,Mockler NM, Ramberg KO, Flood RJ, Crowley PB Biochemistry. 2025 Mar 4;64(5):1092-1098. doi: 10.1021/acs.biochem.4c00729. Epub , 2025 Feb 20. PMID:39977527<ref>PMID:39977527</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9gr4" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ralstonia solanacearum]]
[[Category: Crowley PB]]
[[Category: Flood RJ]]
[[Category: Mockler NM]]
[[Category: Ramberg KO]]