View source for 9gv6


==Structure of TCR in complex with peptide-HLA==
<StructureSection load='9gv6' size='340' side='right'caption='[[9gv6]], [[Resolution|resolution]] 2.77&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9gv6]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9GV6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.77&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9gv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gv6 OCA], [https://pdbe.org/9gv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9gv6 RCSB], [https://www.ebi.ac.uk/pdbsum/9gv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9gv6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A5B8RNS7_HUMAN A0A5B8RNS7_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
T cell receptors (TCRs) recognize specific peptides presented by human leukocyte antigens (HLAs) on the surface of antigen-presenting cells and are involved in fighting pathogens and cancer surveillance. Canonical docking orientation of TCRs to their target peptide-HLAs (pHLAs) is essential for T cell activation, with reverse binding TCRs lacking functionality. TCR binding geometry and molecular interaction footprint with pHLAs are typically obtained by determining the crystal structure. Here, we describe the use of a cross-linking tandem mass spectrometry (XL-MS/MS) method to decipher the binding orientation of several TCRs to their target pHLAs. Cross-linking sites were localized to specific residues and their molecular interactions showed differentiation between TCRs binding in canonical or reverse orientations. Structural prediction and crystal structure determination of two TCR-pHLA complexes validated these findings. The XL-MS/MS method described herein offers a faster and simpler approach for elucidating TCR-pHLA binding orientation and interactions.

Determining T-cell receptor binding orientation and Peptide-HLA interactions using cross-linking mass spectrometry.,Powell T, Karuppiah V, Shaikh SA, Pengelly R, Mai N, Barnbrook K, Sharma A, Harper S, Ebner M, Creese AJ J Biol Chem. 2025 Mar 26;301(5):108445. doi: 10.1016/j.jbc.2025.108445. PMID:40154610<ref>PMID:40154610</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9gv6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Karuppiah V]]