View source for 9gzm


==Cryo-EM structure of the human mitochondrial RNA polymerase transcription initiation complex (POLRMT/TFAM/TFB2M/DNA/RNA) with a 2-mer RNA (pppGpA) and GTP poised for catalysis (pre-IC3)==
<StructureSection load='9gzm' size='340' side='right'caption='[[9gzm]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9gzm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GZM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9GZM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9gzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gzm OCA], [https://pdbe.org/9gzm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9gzm RCSB], [https://www.ebi.ac.uk/pdbsum/9gzm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9gzm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TFB2M_HUMAN TFB2M_HUMAN] S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.<ref>PMID:12068295</ref> <ref>PMID:12897151</ref> <ref>PMID:15526033</ref> <ref>PMID:20410300</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Ajjugal Y]]
[[Category: Das K]]
[[Category: De Wijngaert B]]
[[Category: Goovaerts Q]]
[[Category: Patel SS]]
[[Category: Shen J]]