View source for 9h08


==Crystal structure of Halide methyltransferase from Tulasnella calospora in complex with SAH at room temperature==
<StructureSection load='9h08' size='340' side='right'caption='[[9h08]], [[Resolution|resolution]] 2.36&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9h08]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Tulasnella_calospora_MUT_4182 Tulasnella calospora MUT 4182]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9H08 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9H08 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.36&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9h08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9h08 OCA], [https://pdbe.org/9h08 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9h08 RCSB], [https://www.ebi.ac.uk/pdbsum/9h08 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9h08 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0C3QM78_9AGAM A0A0C3QM78_9AGAM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Enzyme-mediated transfer of methyl groups to specific nucleophilic functions on small metabolites, proteins, and nucleic acids is an essential activity in all known life forms. Most of these transferred methyl groups originate from the one-carbon metabolism through methyl-tetrahydrofolate-dependent methylation of homocysteine, followed by adenosylation of methionine to form the primary methyltransferase cofactor, S-adenosylmethionine (SAM). In this report, we describe a strain of Escherichia coli with a Short-Circuited SAM-Cycle (SCSC) that maintains its SAM pool exclusively by methylating S-adenosylhomocysteine (SAH) using a synthetic methyl donor. Construction of this strain was made possible by the identification of an aryl sulfonate methyl ester as a biocompatible methyl donor and methyltransferases that accept this compound as substrate for in vivo methylation of SAH. We exploited this organism for the optimization of SAH-methylating enzymes by in vivo selection and to produce isotope-labeled natural products. Looking ahead, we anticipate that strains with SCSCs will open new possibilities for methyltransferase biocatalysis, natural product discovery, and bacterial metabolomics.

Short-Circuiting the SAM-Cycle in Escherichia coli.,Li Z, Wen X, Bolotova SB, Seebeck FP J Am Chem Soc. 2025 Dec 11. doi: 10.1021/jacs.5c17370. PMID:41381393<ref>PMID:41381393</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9h08" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Tulasnella calospora MUT 4182]]
[[Category: Bolotova SB]]
[[Category: Seebeck FP]]