View source for 9hjj


==Structure of Trypanosoma cruzi Prolyl Oligopeptidase in the close state==
<StructureSection load='9hjj' size='340' side='right'caption='[[9hjj]], [[Resolution|resolution]] 3.82&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9hjj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9HJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9HJJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.82&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9hjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9hjj OCA], [https://pdbe.org/9hjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9hjj RCSB], [https://www.ebi.ac.uk/pdbsum/9hjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9hjj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q71MD6_TRYCR Q71MD6_TRYCR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, remains a significant global public health concern. Despite its profound health impact in both endemic and non-endemic areas, no vaccine is available, and the existing therapies are outdated, producing severe side effects. The 80 kDa prolyl oligopeptidase of Trypanosoma cruzi (TcPOP) has been identified as a leading candidate for Chagas vaccine development. Here we report the three-dimensional structure of TcPOP in open and closed conformation, at a global resolution of 3.8 and 3.6 A, respectively, determined using single-particle cryo-electron microscopy. Multiple conformations were observed and further characterized using plasmonic optical tweezers and hydrogen-deuterium exchange mass spectrometry. To assess the immunogenic potential of TcPOP, we immunized female mice and evaluated both polyclonal and monoclonal responses against the TcPOP antigen and its homologues. The anti-TcPOP polyclonal response demonstrates invasion blocking properties via parasite lysis. Polyclonal sera were cross-reactive with closely-related POPs but not with human homologues. Collectively, our findings provide structural and functional insights necessary to understand the immunogenicity of TcPOP for future Chagas vaccine development.

Cryo-EM led analysis of open and closed conformations of Chagas vaccine candidate TcPOP.,Batra S, Olmo F, Ragan TJ, Kaplan M, Calvaresi V, Frank AM, Lancey C, Assadipapari M, Ying C, Struwe WB, Hesketh EL, Kelly JM, Barfod L, Campeotto I Nat Commun. 2025 Aug 5;16(1):7164. doi: 10.1038/s41467-025-62068-3. PMID:40764299<ref>PMID:40764299</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9hjj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Trypanosoma cruzi]]
[[Category: Batra S]]
[[Category: Campeotto I]]
[[Category: Hesketh E]]
[[Category: Ragan TJ]]