View source for 9hnk


==Structure of the (6-4) photolyase of Caulobacter crescentus in its oxidizd state - Singal crystal / Synchrotron==
<StructureSection load='9hnk' size='340' side='right'caption='[[9hnk]], [[Resolution|resolution]] 1.59&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9hnk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides Caulobacter vibrioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9HNK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9HNK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.59&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DLZ:1-DEOXY-1-(6,7-DIMETHYL-2,4-DIOXO-3,4-DIHYDROPTERIDIN-8(2H)-YL)-D-RIBITOL'>DLZ</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9hnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9hnk OCA], [https://pdbe.org/9hnk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9hnk RCSB], [https://www.ebi.ac.uk/pdbsum/9hnk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9hnk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9AAF5_CAUVC Q9AAF5_CAUVC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Photolyases repair UV damage to DNA by using absorbed blue light. Within the photolyase/cryptochrome superfamily (PCSf), a major subgroup consists of prokaryotic (6-4) photolyases. These enzymes rely on flavin adenine dinucleotide (FAD) as a catalytic cofactor, besides an ancillary antenna chromophore, and a [4Fe-4S] cluster with yet unknown function. For the prokaryotic 6-4 photolyase of Caulobacter crescentus, we investigated structural changes associated with its different redox states by damage-free crystallography using X-ray free-electron lasers. EPR and optical spectroscopy confirmed redox-dependent structural transitions, including the formation of an oxidized [4Fe-4S](3+) cluster with the dynamic cleavage of a single iron-sulfur bond. Photoreduction to the catalytic FADH(-) state alters the flavin binding site at the proximal aromatic pair Y390/F394 that is part of the electron transport pathway. Upon oxidation, the observable structural transitions of the protein matrix around the [4Fe-4S] cluster may affect DNA binding and are consistent with the much-debated role of the iron-sulfur cluster in DNA-binding proteins for quenching electron holes.

Redox-State-Dependent Structural Changes within a Prokaryotic 6-4 Photolyase.,Wang PH, Hosokawa Y, C Soares J, Emmerich HJ, Fuchs V, Caramello N, Engilberge S, Bologna A, Rosner CJ, Nakamura M, Watad M, Luo F, Owada S, Tosha T, Kang J, Tono K, Bessho Y, Nango E, Pierik AJ, Royant A, Tsai MD, Yamamoto J, Maestre-Reyna M, Essen LO J Am Chem Soc. 2025 Apr 29. doi: 10.1021/jacs.4c18116. PMID:40298610<ref>PMID:40298610</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9hnk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Caulobacter vibrioides]]
[[Category: Large Structures]]
[[Category: Essen L-O]]
[[Category: Maestre-Reyna M]]
[[Category: Po Hsun W]]