View source for 9hta


==Dispersin from Terribacillus saccharophilus Dispts2 in complex with NAG-thiazoline==
<StructureSection load='9hta' size='340' side='right'caption='[[9hta]], [[Resolution|resolution]] 2.17&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9hta]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillaceae Bacillaceae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9HTA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9HTA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.17&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NGT:3AR,5R,6S,7R,7AR-5-HYDROXYMETHYL-2-METHYL-5,6,7,7A-TETRAHYDRO-3AH-PYRANO[3,2-D]THIAZOLE-6,7-DIOL'>NGT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9hta FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9hta OCA], [https://pdbe.org/9hta PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9hta RCSB], [https://www.ebi.ac.uk/pdbsum/9hta PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9hta ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Microorganisms are known to secrete copious amounts of extracellular polymeric substances (EPS) that form complex matrices around the cells to shield them against external stresses, to maintain structural integrity and to influence their environment. Many microorganisms also secrete enzymes that are capable of remodelling or degrading EPS in response to various environmental cues. One key enzyme class is the poly-beta-1,6-linked N-acetyl-D-glucosamine (PNAG)-degrading glycoside hydrolases, of which the canonical member is dispersin B (DspB) from CAZy family GH20. We sought to test the hypothesis that PNAG-degrading enzymes would be present across family GH20, resulting in expansion of the sequence and structural space and thus the availability of PNAGases. Phylogenetic analysis revealed that several microorganisms contain potential DspB-like enzymes. Six of these were expressed and characterized, and four crystal structures were determined (two of which were in complex with the established GH20 inhibitor 6-acetamido-6-deoxy-castanospermine and one with a bespoke disaccharide beta-1,6-linked thiazoline inhibitor). One enzyme expressed rather poorly, which restricted crystal screening and did not allow activity measurements. Using synthetic PNAG oligomers and MALDI-TOF analysis, two of the five enzymes tested showed preferential endo hydrolytic activity. Their sequences, having only 26% identity to the pioneer enzyme DspB, highlight the considerable array of previously unconsidered dispersins in nature, greatly expanding the range of potential dispersin backbones available for societal application and engineering.

Expansion of the diversity of dispersin scaffolds.,Males A, Moroz OV, Blagova E, Munch A, Hansen GH, Johansen AH, Ostergaard LH, Segura DR, Eddenden A, Due AV, Gudmand M, Salomon J, Sorensen SR, Franco Cairo JPL, Nitz M, Pache RA, Vejborg RM, Bhosale S, Vocadlo DJ, Davies GJ, Wilson KS Acta Crystallogr D Struct Biol. 2025 Mar 1;81(Pt 3):130-146. doi: , 10.1107/S205979832500110X. Epub 2025 Feb 28. PMID:40019001<ref>PMID:40019001</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9hta" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillaceae]]
[[Category: Large Structures]]
[[Category: Bhosale S]]
[[Category: Blagova E]]
[[Category: Davies GJ]]
[[Category: Due AV]]
[[Category: Eddenden A]]
[[Category: Franco Cairo JPL]]
[[Category: Gudmand M]]
[[Category: Hansen GH]]
[[Category: Johansen AH]]
[[Category: Males A]]
[[Category: Moroz OV]]
[[Category: Munch A]]
[[Category: Ostergaard LH]]
[[Category: Pache RA]]
[[Category: Salomon J]]
[[Category: Segura DR]]
[[Category: Sorensen SR]]
[[Category: Vejborg RM]]
[[Category: Vocadlo D]]
[[Category: Wilson KS]]