View source for 9ilo


==Crystal structure of CTB10-M4==
<StructureSection load='9ilo' size='340' side='right'caption='[[9ilo]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9ilo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cercospora_sp._JNU001 Cercospora sp. JNU001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9ILO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9ILO FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.21&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PBF:PARA-(BENZOYL)-PHENYLALANINE'>PBF</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ilo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ilo OCA], [https://pdbe.org/9ilo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ilo RCSB], [https://www.ebi.ac.uk/pdbsum/9ilo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ilo ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A977K7H6_9PEZI A0A977K7H6_9PEZI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The creation of enzymes with abiological abilities offers exciting opportunities to access new-to-nature biocatalysis beyond that found in nature. Here, we repurpose a novel protein scaffold, CTB10, as an artificial photoenzyme through genetic code expansion. It enables catalytic deracemization of cyclopropane, a process that remains inaccessible to traditional biocatalysis due to its thermodynamically unfavorable nature. Following structural optimization through directed evolution, a broad substrate scope with high enantioselectivities is achieved. Furthermore, the crystal structure of the CTB10-based photoenzyme-substrate complex well demonstrates how the catalytic chiral cavity is sculpted to promote efficient and selective light-enabled deracemization. Therefore, this study unlocks the potential for achieving challenging deracemization through biocatalysis.

Light-Driven Deracemization by a Designed Photoenzyme.,Li M, Zhang Y, Fu K, Deng Z, Yuan Z, Luo Z, Rao Y J Am Chem Soc. 2025 Apr 23;147(16):13190-13199. doi: 10.1021/jacs.4c16521. Epub , 2025 Apr 12. PMID:40219972<ref>PMID:40219972</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9ilo" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cercospora sp. JNU001]]
[[Category: Large Structures]]
[[Category: Fu K]]
[[Category: Rao YJ]]