View source for 9ixa


==Cryo-EM structure of chikungunya virus glycoprotein E1-E2 with C34 Fab.==
<StructureSection load='9ixa' size='340' side='right'caption='[[9ixa]], [[Resolution|resolution]] 3.11&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9ixa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chikungunya_virus Chikungunya virus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9IXA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9IXA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.11&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ixa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ixa OCA], [https://pdbe.org/9ixa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ixa RCSB], [https://www.ebi.ac.uk/pdbsum/9ixa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ixa ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C8YZ73_CHIKV C8YZ73_CHIKV] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that causes febrile illness and acute or chronic arthritis. Most therapeutics are still in the pre-clinical stage. In this study, we report the isolation of two neutralizing antibodies, C34 and C37, from a convalescent patient and investigate their mechanisms of action. Both C34 and C37 exhibit high neutralizing activities in vitro and demonstrate protective effects against CHIKV in a female mouse model. Our functional and structural studies reveal a mechanism that inhibits multiple stages of the virus infection cycle. Both antibodies bind with high affinity to an epitope spanning E2, E1, and the connecting beta-strands, facilitating intra- and inter-virion crosslinking. Cryo-EM structures additionally identify a minor patch located beneath the E3 binding site on E2, which is allosterically exposed upon E3 dissociation during virus maturation. Functional and structural data further suggest that binding to the CHIKV receptor, Mxra8, is obstructed due to a clash between the antibodies and the stalk region of Mxra8. Our results highlight the potential of antibody-based therapeutics against CHIKV and elucidate the mechanisms of monoclonal antibody protection.

Neutralizing antibodies against Chikungunya virus and structural elucidation of their mechanism of action.,Han X, Ji C, Tian S, Wang F, Cao GP, Li D, Duan X, Tong Z, Qi J, Wang Q, Huang Q, Zhan BD, Gao GF, Yan J Nat Commun. 2025 Nov 3;16(1):9682. doi: 10.1038/s41467-025-64687-2. PMID:41184282<ref>PMID:41184282</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9ixa" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Chikungunya virus]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Gao FG]]
[[Category: Han X]]
[[Category: Ji C]]
[[Category: Tian S]]
[[Category: Wang F]]
[[Category: Yan J]]