View source for 9jjd


==Structural analysis of autophagy-related protein 8a in Drosophila melanogaster==
<StructureSection load='9jjd' size='340' side='right'caption='[[9jjd]], [[Resolution|resolution]] 1.36&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9jjd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9JJD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9JJD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.36&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9jjd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9jjd OCA], [https://pdbe.org/9jjd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9jjd RCSB], [https://www.ebi.ac.uk/pdbsum/9jjd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9jjd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9W2S2_DROME Q9W2S2_DROME] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Autophagy-associated protein 8 (ATG8) is essential for autophagy and organismal growth and development. In this study, we successfully resolved the crystal structure of Drosophila melanogaster (D. melanogaster) ATG8a (DmATG8a) at 1.36 A resolution. Being distinct from previously characterized ATG8 homologues, DmATG8a (121 residues) adopts a unique fold comprising five alpha-helices and four beta-folding strands, in contrast to the canonical four alpha-helices and four beta-folding strands observed in other ATG8 proteins. DmATG8a features two active cavities: hydrophobic pocket 1 (HP1) and hydrophobic pocket 2 (HP2), which are essential for the normal physiological function of ATG8. Indole and its analogs can bind specifically with HP1. Microscale thermophoresis results demonstrated a strong affinity of 6-fluoroindole with DmATG8a (3.54 mumol/L), but no affinity with the DmATG8a(K48A) mutant, suggesting that Lys48 is critical in binding 6-fluoroindole probably via a hydrogen bond interaction. The half-maximum lethal concentration (LC(50)) of 6-fluoroindole against D. melanogaster adult flies was 169 mug/mL. Our findings establish DmATG8a as a promising target for developing indole-based insecticides.

Crystal Structure of Autophagy-Associated Protein 8 at 1.36 A Resolution and Its Inhibitory Interactions with Indole Analogs.,Zhang S, Luo X, Yuan X, Wu D, Liu J, Zhao K, Xu Y, Zhou J, Li X, Li QX J Agric Food Chem. 2025 Mar 26;73(12):7111-7120. doi: 10.1021/acs.jafc.4c11205. , Epub 2025 Mar 11. PMID:40066832<ref>PMID:40066832</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9jjd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Li XY]]
[[Category: Liu J]]
[[Category: Luo X]]
[[Category: Wu DX]]
[[Category: Zhang SQ]]