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==Crystal structure of the Staphylococcus aureus GTPase Era in complex with GDP==
<StructureSection load='9jkp' size='340' side='right'caption='[[9jkp]], [[Resolution|resolution]] 2.76&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9jkp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_NCTC_8325 Staphylococcus aureus subsp. aureus NCTC 8325]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9JKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9JKP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.76&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9jkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9jkp OCA], [https://pdbe.org/9jkp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9jkp RCSB], [https://www.ebi.ac.uk/pdbsum/9jkp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9jkp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ERA_STAA8 ERA_STAA8] An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism.[HAMAP-Rule:MF_00367]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
GTPase Era from Staphylococcus aureus belongs to the TRAFAC superfamily of the TrmE-Era-EngA-EngB-Septin-like GTPases class and plays a significant role in the vital activity of this pathogenic microorganism as a maturation factor of the 30S ribosome subunit. However, the functions of this protein are not fully understood, making it a promising object for further study. Here, the 2.76 A resolution crystal structure of Staphylococcus aureus Era in complex with GDP is presented. Structural comparison with other GTP-bound and GDP-bound homologous proteins, GTPase domain and the KH domain revealed a mutual orientation in S. aureus which has not been described before. The GDP-bound Era structure presented here will facilitate efforts to elucidate its interactions with its regulators and lay the foundation for a structure-based search for specific inhibitors.

Crystal structure of the GDP-bound GTPase Era from Staphylococcus aureus.,Klochkova E, Biktimirov A, Islamov D, Belousov A, Validov S, Yusupov M, Usachev K Biochem Biophys Res Commun. 2024 Nov 26;735:150852. doi: , 10.1016/j.bbrc.2024.150852. Epub 2024 Oct 18. PMID:39432921<ref>PMID:39432921</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9jkp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus subsp. aureus NCTC 8325]]
[[Category: Belousov A]]
[[Category: Biktimirov A]]
[[Category: Islamov D]]
[[Category: Klochkova E]]
[[Category: Usachev K]]
[[Category: Yusupov M]]