View source for 9jry


==Crystal structure of FiDCB, a dual-cysteine cyanobacterial phytochrome of Fischerella sp. PCC 9605==
<StructureSection load='9jry' size='340' side='right'caption='[[9jry]], [[Resolution|resolution]] 3.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9jry]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fischerella_sp._PCC_9605 Fischerella sp. PCC 9605]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9JRY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9JRY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.35&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLR:3-[5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-2-[[5-[(Z)-(3-ethenyl-4-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1H-pyrrol-2-yl]methyl]-4-methyl-1H-pyrrol-3-yl]propanoic+acid'>BLR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9jry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9jry OCA], [https://pdbe.org/9jry PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9jry RCSB], [https://www.ebi.ac.uk/pdbsum/9jry PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9jry ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Previous studies have identified three families of knotted phytochrome photoreceptors in cyanobacteria. We describe a fourth type: 'hybrid' phytochromes with putative bilin-binding cysteine residues in both their N-terminal 'knot' extensions and cGMP-phosphodiesterase/adenylate cyclase/FhlA (GAF) domains, which we designate as dual-cysteine bacteriophytochromes (DCBs). Recombinant expression of DCBs in Escherichia coli yields photoactive phycocyanobilin (PCB) adducts with red/far-red photocycles similar to those of the GAF-Cys-containing cyanobacterial phytochromes (Cph1s). Incorporation of the PCB precursor, biliverdin IXalpha (BV), gave multiple populations, one of which appears similar to those of cyanobacterial bacteriophytochromes (cBphPs). A crystal structure of FiDCB bound to BV exhibits two thioether linkages between the GAF- and 'PAS-knot'-Cys residues and the C3(1) and C3(2) atoms of BV. When expressed in Synechocystis sp. PCC 6803, DCBs incorporate PCB rather than BV. DCBs can be converted to photoactive cBphP-, Cph1-, and tandem-cysteine cyanobacterial phytochrome (TCCP) analogs by removal and/or addition of a cysteine residue by site-directed mutagenesis. This structural plasticity contrasts with our inability to generate functional photosensor analogs by analogous site-directed mutagenesis of TCCP and Cph1 representatives. Phylogenetic analysis demonstrates that DCBs do not form a monophyletic clade and also suggest that Cph1 and TCCP families independently emerged from different lineages of cBphPs, possibly via DCB intermediates.

Dual-Cys bacteriophytochromes: intermediates in cyanobacterial phytochrome evolution?,Yang HW, Song JY, Song JJ, Kim YW, Rockwell NC, Kim W, Kim H, Lagarias JC, Park YI FEBS J. 2025 Jan 13. doi: 10.1111/febs.17395. PMID:39801362<ref>PMID:39801362</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 9jry" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Fischerella sp. PCC 9605]]
[[Category: Large Structures]]
[[Category: Park Y-I]]
[[Category: Song J-J]]
[[Category: Yang HW]]