View source for 9jwg


==Cryo-EM Focused Refined Map of Human RNF213 E3 module and IpaH1.4 LRR domain==
<StructureSection load='9jwg' size='340' side='right'caption='[[9jwg]], [[Resolution|resolution]] 3.47&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9jwg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9JWG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9JWG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.47&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9jwg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9jwg OCA], [https://pdbe.org/9jwg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9jwg RCSB], [https://www.ebi.ac.uk/pdbsum/9jwg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9jwg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0A0MTR7_HUMAN A0A0A0MTR7_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ubiquitination plays vital roles in modulating pathogen-host cell interactions. RNF213, a E3 ligase, can catalyze the ubiquitination of lipopolysaccharide (LPS) and is crucial for antibacterial immunity in mammals. Shigella flexneri, an LPS-containing pathogenic bacterium, has developed mechanisms to evade host antibacterial defenses during infection. However, the precise strategies by which S. flexneri circumvents RNF213-mediated antibacterial immunity remain poorly understood. Here, through comprehensive biochemical, structural and cellular analyses, we reveal that the E3 effector IpaH1.4 of S. flexneri can directly target human RNF213 via a specific interaction between the IpaH1.4 LRR domain and the RING domain of RNF213, and mediate the ubiquitination and proteasomal degradation of RNF213 in cells. Furthermore, we determine the cryo-EM structure of human RNF213 and the crystal structure of the IpaH1.4 LRR/RNF213 RING complex, elucidating the molecular mechanism underlying the specific recognition of RNF213 by IpaH1.4. Finally, our cell based functional assays demonstrate that the targeting of host RNF213 by IpaH1.4 promotes S. flexneri proliferation within infected cells. In summary, our work uncovers an unprecedented strategy employed by S. flexneri to subvert the key host immune factor RNF213, thereby facilitating bacterial proliferation during invasion.

Shigella effector IpaH1.4 subverts host E3 ligase RNF213 to evade antibacterial immunity.,Zhou X, Zhang H, Wang Y, Wang D, Lin Z, Zhang Y, Tang Y, Liu J, Yao YF, Zhang Y, Pan L Nat Commun. 2025 Apr 1;16(1):3099. doi: 10.1038/s41467-025-58432-y. PMID:40164614<ref>PMID:40164614</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9jwg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Shigella flexneri]]
[[Category: Zhang H]]