View source for 9jwk


==Cryo-EM structure of FrCas9 in complex with sgRNA and 26-nt TS and 4-nt NTS substrates==
<StructureSection load='9jwk' size='340' side='right'caption='[[9jwk]], [[Resolution|resolution]] 3.46&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9jwk]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Faecalibaculum_rodentium Faecalibaculum rodentium] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9JWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9JWK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.46&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9jwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9jwk OCA], [https://pdbe.org/9jwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9jwk RCSB], [https://www.ebi.ac.uk/pdbsum/9jwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9jwk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1Q9YNK3_9FIRM A0A1Q9YNK3_9FIRM] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.[HAMAP-Rule:MF_01480]
__TOC__
</StructureSection>
[[Category: Faecalibaculum rodentium]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Chen SD]]
[[Category: Liu SQ]]
[[Category: Yang M]]