View source for 9kbi


==The structure of B19V NS1_2-570/dsDNA/AMPPNP==
<StructureSection load='9kbi' size='340' side='right'caption='[[9kbi]], [[Resolution|resolution]] 3.43&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9kbi]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_parvovirus_B19 Human parvovirus B19]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9KBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9KBI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.43&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9kbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9kbi OCA], [https://pdbe.org/9kbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9kbi RCSB], [https://www.ebi.ac.uk/pdbsum/9kbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9kbi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/I7BP20_PAVHB I7BP20_PAVHB] Multifunctional protein essential for viral DNA replication, which cooperatively interacts with the viral DNA origin of replication and transactivates several promoters including the viral p6 promoter. Binds the origin of replication and performs an endonucleolytic nick within a conserved sequence in the viral genome, thereby initiating the rolling circle replication (RCR). Participates in the transcriptional regulation the viral p6 promoter that regulates all viral transcripts and the cellular CDN1A or IL6 promoters. Transactivates several host promoters some of which induce the S cell cycle phase for the production of host replicative proteins. Up-regulates the expression of host E2F4 and E2F5 and interacts with both these factors thereby inhibiting the host cell cycle G2/M transition. This arrest promotes apoptosis for viral release.[ARBA:ARBA00055704]
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</StructureSection>
[[Category: Human parvovirus B19]]
[[Category: Large Structures]]
[[Category: Gan J]]
[[Category: Zhang Y]]