View source for 9kgb


==Crystal structure of tolypothrichol synthase==
<StructureSection load='9kgb' size='340' side='right'caption='[[9kgb]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9kgb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Scytonema Scytonema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9KGB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9KGB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9kgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9kgb OCA], [https://pdbe.org/9kgb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9kgb RCSB], [https://www.ebi.ac.uk/pdbsum/9kgb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9kgb ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
In recent years, genome mining in cyanobacteria has revealed abundant gene clusters related to natural product biosynthesis. However, only a few terpene synthases (TSs) have been identified from this bacterial phylum. Pfam profiles, such as PF03936 and PF19086, which are frequently used for TS retrieval, are built from plant, bacterial, and fungal TSs. Herein, we constructed a new hidden Markov model (HMM) specific to bacterial TSs on the basis of 110 bacterial TSs experimentally validated in recent years. Using this model, we identified a pair of diterpene synthases, Cpt11 (class II TS) and Cts11 (class I TS), in the cyanobacterium Scytonema tolypothrichoides. In vitro experiments demonstrated that Cpt11 catalyzes the formation of syn-copalyl diphosphate from geranylgeranyl diphosphate and that Cts11 subsequently converts syn-copalyl diphosphate into a rare 6,6,7-tricyclic diterpene alcohol. Its biosynthesis was established through isotope labeling experiments, which revealed a unique sequence of a 1,6-proton shift and ring expansion to a seven-membered ring. We solved the crystal structure of Cts11 at a resolution of 1.76 A. Additionally, via site-directed mutagenesis experiments, we identified two amino acid residues whose exchanges affected the formation of the original diterpene alcohol, leading to the formation of two new compounds: a 6,6,7-tricyclic diterpene hydrocarbon and another 6,6,6-tricyclic diterpene alcohol. A BLAST search revealed several sequences that shared over 70% identity with Cts11 from cyanobacteria that could produce diverse diterpenes. This study demonstrates the potential for cyanobacteria to produce unprecedented terpenoids and lays the foundation for studying the physiological activities of terpenoids in cyanobacteria.

Mechanistic Characterization of Diterpene Synthase Pairs for Tricyclic Diterpenes from Cyanobacteria.,Yu J, Shiraishi T, Taizoumbe KA, Karasuno Y, Yoshida A, Nishiyama M, Dickschat JS, Kuzuyama T J Am Chem Soc. 2025 Mar 27. doi: 10.1021/jacs.4c16710. PMID:40143759<ref>PMID:40143759</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 9kgb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Scytonema]]
[[Category: Kuzuyama T]]
[[Category: Shiraishi T]]
[[Category: Yoshida A]]
[[Category: Yu J]]