View source for 9kou


==CryoEM structure of osPHT1-11 at pH 5.0==
<StructureSection load='9kou' size='340' side='right'caption='[[9kou]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9kou]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9KOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9KOU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9kou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9kou OCA], [https://pdbe.org/9kou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9kou RCSB], [https://www.ebi.ac.uk/pdbsum/9kou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9kou ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PT111_ORYSJ PT111_ORYSJ] Symbiosis-specific regulated inorganic phosphate (Pi) transporter. Probably involved in symbiosis-mediated Pi uptake in roots colonized by myccorhizal fungi.<ref>PMID:12271140</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phosphorus is an essential macronutrient for plants, primarily absorbed from the soil as inorganic phosphate (Pi) through root-located Pi transporters. Despite decades of research into these transporters as targets for developing Pi-efficient crops, their mechanisms for Pi import remain poorly understood. Here, we present the cryo-electron microscopy (cryo-EM) structures of the rice Pi importer OsPHT1;11 in both Pi-bound and unbound forms, characterize its conformational dynamics, and demonstrate how these dynamics contribute to its transport function. Pi is recognized through conserved residues found in plants, with its translocation facilitated by a typical alternating-access mechanism. Single-molecule fluorescence resonance energy transfer (smFRET) analyses show that this transporter undergoes dynamic conformational fluctuations, which are differentially linked to its Pi transport capability, with a predominance of extracellular open conformations favoring Pi transport, while more populated intracellular open conformations hinder it. These findings highlight key conformational determinants of transport activity and provide mechanistic insights into Pi uptake in plants.

Cryo-EM structure and dynamic basis of phosphate uptake by PHT1 in rice.,Du Z, Guan Z, Liu H, Zhang J, He H, Zheng Z, Zhang W, Jiang L, Zuo J, Liu Y, Wan B, Tu H, Dong F, Lai X, Xiong L, Yin P, Xue S, Chen Y, Liu Z Dev Cell. 2025 Sep 25:S1534-5807(25)00541-6. doi: 10.1016/j.devcel.2025.09.003. PMID:41005295<ref>PMID:41005295</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9kou" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Oryza sativa Japonica Group]]
[[Category: Du ZM]]
[[Category: Guan ZY]]
[[Category: Liu Z]]