View source for 9kpl


==Crystal structure of T. rubripes Mincle with glucose==
<StructureSection load='9kpl' size='340' side='right'caption='[[9kpl]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9kpl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Takifugu_rubripes Takifugu rubripes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9KPL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9KPL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9kpl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9kpl OCA], [https://pdbe.org/9kpl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9kpl RCSB], [https://www.ebi.ac.uk/pdbsum/9kpl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9kpl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A3B5KEF3_TAKRU A0A3B5KEF3_TAKRU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Our bodies are continuously exposed to injurious insults by infection and tissue damage, which are primarily sensed by innate immune receptors to maintain homeostasis. Among such receptors is macrophage-inducible C-type lectin (Mincle, gene symbol CLEC4E), a member of the C-type lectin receptor (CLR) family, which functions as an immune sensor for both pathogens and damaged self. To monitor these injurious stimuli, Mincle recognizes disaccharide-based pathogen-derived glycolipids and monosaccharide-based intracellular metabolites, such as beta-glucosylceramide. Mincle is well-conserved among mammals; however, there are questions that remain unclear, such as from which lower vertebrate did it arise and whether the original ligand was self or non-self. Here, we found homologues of Mincle and its signaling subunit Fc receptor gamma chain (FcRgamma) in lower vertebrates, such as reptiles, amphibians, and fishes. The crystal structure of a Mincle homologue revealed that fish Mincle possesses a narrower sugar-binding pocket than that of mammalian Mincle, and accommodates only monosaccharide moieties. These results suggest that Mincle may have evolved from a self-recognizing receptor, and its sugar-binding pocket widened during evolution, presumably to adapt to disaccharide-based glycolipids derived from life-threatening pathogens.

Phylogenetic and structural insights into the origin of C-type lectin Mincle in vertebrates.,Ito T, Guenther C, Ishikawa E, Yabuki T, Nagae M, Nakatani Y, Yamasaki S Immunogenetics. 2025 Mar 22;77(1):18. doi: 10.1007/s00251-025-01375-x. PMID:40119899<ref>PMID:40119899</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9kpl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Takifugu rubripes]]
[[Category: Ito T]]
[[Category: Nagae M]]
[[Category: Yamasaki S]]