View source for 9lbq


==Cryo-EM structure of the Retron-Eco8 complex==
<StructureSection load='9lbq' size='340' side='right'caption='[[9lbq]], [[Resolution|resolution]] 2.94&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9lbq]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9LBQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9LBQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.94&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9lbq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9lbq OCA], [https://pdbe.org/9lbq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9lbq RCSB], [https://www.ebi.ac.uk/pdbsum/9lbq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9lbq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RT8_ECOLX RT8_ECOLX] Reverse transcriptase (RT) component of antiviral defense system retron Eco8, composed of this RT, the following endonuclease and a non-coding RNA (ncRNA) encoded between them. Expression of retron Eco8 confers protection against bacteriophages T4, T6, T7 and SECphi4, SECphi6 and SECphi18. At multiplicity of infection (MOI) of 0.02 cultures slow growth when infected with SECphi4 but do not collapse, at MOI 2 cultures collapse. Responsible for synthesis of msDNA (a branched molecule with RNA linked by a 2',5'-phosphodiester bond to ssDNA). The retron transcript serves as primer (from a conserved internal G residue) and template for the reaction, and codes for the RT.<ref>PMID:33157039</ref> 
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Ji CG]]
[[Category: Jia N]]
[[Category: Zhang JT]]