View source for 9lfn


==Cryo-EM structure of the rice isoamylase ISA1-ISA2 heterocomplex incubated with Amylopectin==
<StructureSection load='9lfn' size='340' side='right'caption='[[9lfn]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9lfn]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9LFN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9LFN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1EKO:(2~{R},3~{S},4~{R},5~{R},6~{S})-6-(hydroxymethyl)oxane-2,3,4,5-tetrol'>A1EKO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9lfn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9lfn OCA], [https://pdbe.org/9lfn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9lfn RCSB], [https://www.ebi.ac.uk/pdbsum/9lfn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9lfn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ISOA1_ORYSJ ISOA1_ORYSJ] Starch-debranching enzyme involved in amylopectin biosynthesis in endosperm. Functions by removing excess branches or improper branches that interfere with the formation of double helices of the cluster chains of amylopectin and crystallization of starch (PubMed:10517831, PubMed:15618430, PubMed:16953433, PubMed:21436381). Works as ISA1 homooligomer or together with ISA2 as heterooligomer. The heterooligomer ISA1 and ISA2 possesses higher affinity than the ISA1 homooligomer for various branched polyglucans in vitro, but no marked differences exist in chain preferences for debranching of amylopectin and phytoglycogen between these forms (PubMed:16953433, PubMed:21436381).<ref>PMID:10517831</ref> <ref>PMID:15618430</ref> <ref>PMID:16953433</ref> <ref>PMID:21436381</ref> 
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Oryza sativa Japonica Group]]
[[Category: Guan ZY]]
[[Category: Yan JJ]]