View source for 9ll5


==Crystal structure of the Helicobacter pylori copper resistance determinant CrdA in complex with silver ions in space group P1==
<StructureSection load='9ll5' size='340' side='right'caption='[[9ll5]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9ll5]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori Helicobacter pylori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9LL5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9LL5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AG:SILVER+ION'>AG</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ll5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ll5 OCA], [https://pdbe.org/9ll5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ll5 RCSB], [https://www.ebi.ac.uk/pdbsum/9ll5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ll5 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Helicobacter pylori is a bacterium that colonizes the stomach and causes gastric disorders in humans. For successful colonization in the harsh gastric environment, H. pylori employs various homeostatic mechanisms in response to environmental factors, such as protons and copper ions. Copper levels should be maintained below toxicity in the cell while remaining above the threshold required for biological functions. Copper resistance determinant A (CrdA) is a putative copper chaperone protein that contributes to copper homeostasis in H. pylori. To provide insight into CrdA-mediated copper homeostasis, we analyzed the interaction of CrdA with Cu(I) or Cu(II) ions through biochemical and mutational studies and determined the crystal structures of CrdA alone and in complex with a Cu(I) mimic, Ag(I). CrdA exhibited a binding preference for Cu(I) and Ag(I) ions over Cu(II) ions. CrdA forms a Greek key beta-barrel structure with a unique protruding methionine-rich motif whose methionine residues coordinate two Ag(I) ions. In particular, the CrdA residue M69 plays a key role in Cu(I) recognition, even at the low pH found in the stomach where H. pylori resides. Furthermore, our structure-based comparative analysis suggests that CrdA has evolved a unique Cu(I) recognition mechanism that has not been observed for other Cu(I) chaperone proteins. Our findings on the specific interaction of CrdA with Cu(I) ions would provide a new avenue for developing H. pylori-targeting antibacterial drugs.

Structural basis of Cu(I) ion recognition by the Helicobacter pylori copper resistance determinant CrdA.,Ki DU, Oh HB, Cho HY, Song WS, Yoon SI FEBS J. 2025 Oct 28. doi: 10.1111/febs.70305. PMID:41147750<ref>PMID:41147750</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 9ll5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Helicobacter pylori]]
[[Category: Large Structures]]
[[Category: Cho HY]]
[[Category: Ki DW]]
[[Category: Oh HB]]
[[Category: Song WS]]
[[Category: Yoon SI]]