View source for 9lol


==Cryo-EM structure of human MON1A-CCZ1-RAB7A==
<StructureSection load='9lol' size='340' side='right'caption='[[9lol]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9lol]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9LOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9LOL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9lol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9lol OCA], [https://pdbe.org/9lol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9lol RCSB], [https://www.ebi.ac.uk/pdbsum/9lol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9lol ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CCZ1B_HUMAN CCZ1B_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Autophagy is a fundamental cellular process, conserved across species from yeast to mammals, that plays a crucial role in maintaining cellular homeostasis. The functionally conserved MON1-CCZ1 (MC1) complex serves as a guanine nucleotide exchange factor (GEF) for the RAB GTPase RAB7A and is indispensable for directing RAB7A recruitment to autophagosome or lysosomal membranes. Despite its critical role, the precise molecular mechanism underlying the assembly of the human MON1A-CCZ1 (HsMC1) complex and its specific GEF activity towards RAB7A has remained unclear. In this study, we report the high-resolution cryo-electron microscopy (cryo-EM) structure of the HsMC1 GEF domain in a complex with the nucleotide-free RAB7A(N125I) at 2.85 A resolution. Our structural data demonstrate that engagement with the HsMC1 complex induces marked conformational shifts in the phosphate-binding loop (P-loop) and Switch I/II regions of RAB7A. A striking feature of this complex is the direct interaction between the P-loop of RAB7A and CCZ1, a structural detail not previously observed. Furthermore, biochemical assays targeting residues within Interface I or II of the HsMC1-RAB7A complex highlight their critical role in mediating the interaction and suggest a unique mechanism for nucleotide exchange facilitated by the HsMC1 complex. These findings provide novel molecular insights into the functional mechanisms of the HsMC1-RAB7A complex, offering a robust structural framework to inform future investigations into disease-related targets and therapeutic development.

Cryo-EM structure of the human MON1A-CCZ1-RAB7A complex provides insights into nucleotide exchange mechanism.,Li X, Li D, Tang D, Huang X, Bao H, Wang J, Qi S Life Metab. 2025 May 26;4(5):loaf017. doi: 10.1093/lifemeta/loaf017. eCollection , 2025 Oct. PMID:40979223<ref>PMID:40979223</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9lol" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Li D]]
[[Category: Li X]]
[[Category: Qi S]]
[[Category: Tang D]]
[[Category: Wang J]]