View source for 9lpb


==Terpene cyclase AriE mutant-D128A==
<StructureSection load='9lpb' size='340' side='right'caption='[[9lpb]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9lpb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Amycolatopsis_arida Amycolatopsis arida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9LPB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9LPB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9lpb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9lpb OCA], [https://pdbe.org/9lpb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9lpb RCSB], [https://www.ebi.ac.uk/pdbsum/9lpb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9lpb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1I5P1T3_9PSEU A0A1I5P1T3_9PSEU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Eunicellane diterpenoids, with over 360 known examples characterized by a cis- or trans-6/10-bicyclic carbon skeleton, exhibit diverse biological activities. To date, five eunicellane cyclases (ECs) have been reported: cis-ECs (Bnd4, CpDTS2 and EcTPS1) and trans-ECs (AlbS and MicA), yet the structural basis and molecular mechanisms governing their distinct cis- or trans-cyclization modes remain unsolved. Here, we present the crystal structure of a cis-EC AriE from Amycolatopsis arida, which catalyzes geranylgeranyl diphosphate to form cis-6/10-fused bicyclic benditerpe-2,6,15-triene (1) in aridacin biosynthesis. Through surface entropy reduction, we obtained the 1.87 A resolution structure of AriED128A. Structure-guided mutagenesis identified W73 as a key regulatory hotspot controlling product diversity, with its variants generating diverse diterpene products. Y203A converted AriE into a highly specific (-)-(R)-cembrene A synthase. Our findings provide the first structural insights into this type of terpene synthase and demonstrate the feasibility of engineering EC variants for selective product formation.

Crystal Structure and Catalytic Mechanism of the cis-Eunicellane Cyclase AriE.,Li FR, Yang Q, He J, Sun X, Pan X, Xu HM, Rudolf JD, Dong LB Chemistry. 2025 Apr 23:e202500012. doi: 10.1002/chem.202500012. PMID:40265331<ref>PMID:40265331</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9lpb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Amycolatopsis arida]]
[[Category: Large Structures]]
[[Category: Dong LB]]
[[Category: He JY]]
[[Category: Li FR]]
[[Category: Pan XM]]
[[Category: Rudolf JD]]
[[Category: Sun XR]]
[[Category: Xu HM]]
[[Category: Yang Q]]