View source for 9lsd


==Crystal structure of a polyketide cyclase FasU from Streptomyces kanamyceticus==
<StructureSection load='9lsd' size='340' side='right'caption='[[9lsd]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9lsd]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_kanamyceticus Streptomyces kanamyceticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9LSD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9LSD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AE3:2-(2-ETHOXYETHOXY)ETHANOL'>AE3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9lsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9lsd OCA], [https://pdbe.org/9lsd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9lsd RCSB], [https://www.ebi.ac.uk/pdbsum/9lsd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9lsd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A5J6G7S2_STRKN A0A5J6G7S2_STRKN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Aromatic polyketides are an important class of natural products with various bioactivities, and their structural diversity arises from modifications to their aromatic frameworks. In this study, we identify a stereoselective aromatase/cyclase (ARO/CYC) FasU, which is responsible for forming the axial chiral biphenyl framework in fasamycin. FasU catalyzes sequential decarboxylation and cyclization/aromatization with strict S-stereospecificity on a previously unidentified biosynthetic intermediate. Through crystal structure analysis and site-directed mutagenesis, we reveal the enzyme's substrate binding mode, stereospecificity, and the key residues involved in catalysis. This discovery introduces a novel class of ARO/CYC enzymes in type II polyketide biosynthesis, advancing the development of biocatalysts for chiral aromatic polyketides.

A Stereoselective Decarboxylative Aromatase/Cyclase Directs the Biosynthesis of an Axially Chiral Biphenyl Framework in Fasamycin.,Jiang K, Zhu C, Yan X, Li G, Lin Z, Deng Z, Luo S, Qu X J Am Chem Soc. 2025 Feb 19;147(7):5596-5601. doi: 10.1021/jacs.4c18376. Epub 2025 , Feb 6. PMID:39910892<ref>PMID:39910892</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9lsd" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces kanamyceticus]]
[[Category: Luo S]]
[[Category: Zhu C]]