View source for 9luk


==Crystal structure of Switchbody based on anti-osteocalcin antibody KTM219 Fab==
<StructureSection load='9luk' size='340' side='right'caption='[[9luk]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9luk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9LUK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9LUK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9luk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9luk OCA], [https://pdbe.org/9luk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9luk RCSB], [https://www.ebi.ac.uk/pdbsum/9luk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9luk ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
An enzyme switch, termed "Switchbody", is developed by fusing an antibody with a fragment of a split enzyme for the precise regulation of enzyme activity in response to an antigen. A luciferase-based Switchbody is engineered by fusing the NanoLuc luciferase fragment HiBiT to the N-terminus of an antibody. The enzyme activity of the Switchbody increases upon the addition of an antigen in a dose-dependent manner in the presence of the complementary fragment LgBiT and its substrate furimazine, demonstrating the potential of the luciferase-based Switchbody as a biosensor. As its working principle, ELISA shows that the interaction between HiBiT and LgBiT is facilitated by antigen binding. Moreover, X-ray crystallography and NMR reveal the heterogeneous trapped state of the HiBiT region and an increasing motility of HiBiT region upon antigen binding, respectively. MD simulations and luminescence measurements show that antigen disrupted the trapping of HiBiT in the antibody, enabling its release. By applying this "Trap and Release" principle to Protein M, an antibody-binding protein, label-free IgG antibodies are successfully converted into bioluminescent Switchbodies. This adaptable Switchbody platform has the potential to expand switching technology beyond luciferase to various other enzymes in the future.

Switchbody, an Antigen-Responsive Enzyme Switch Based on Antibody and Its Working Principle.,Yasuda T, Ueno Y, Taguchi M, Tochio N, Yagi H, Yazaki S, Arai R, Zhu B, Kigawa T, Ueda H, Kitaguchi T Adv Sci (Weinh). 2025 Sep 15:e08422. doi: 10.1002/advs.202508422. PMID:40953287<ref>PMID:40953287</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9luk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Arai R]]
[[Category: Kitaguchi T]]
[[Category: Ueda H]]
[[Category: Yasuda T]]
[[Category: Yazaki S]]