View source for 9lw8


==Bottom cap of bacteriophage Mycofy1 mature head (C5 symmetry)==
<StructureSection load='9lw8' size='340' side='right'caption='[[9lw8]], [[Resolution|resolution]] 3.53&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9lw8]] is a 53 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_phage_Mycofy1 Mycolicibacterium phage Mycofy1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9LW8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9LW8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.53&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9lw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9lw8 OCA], [https://pdbe.org/9lw8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9lw8 RCSB], [https://www.ebi.ac.uk/pdbsum/9lw8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9lw8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q854Z2_9CAUD Q854Z2_9CAUD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mycobacteriophages show promise in treating antibiotic-resistant mycobacterial infections. Here, we isolated Mycofy1, a mycobacteriophage, using M. smegmatis as a host. Cryo-EM analysis revealed that Mycofy1 possesses a prolate head and a long non-contractile tail. We determined structures of its head, head-to-tail interface, terminator, and tail tube to resolutions of approximately 3.5 A. Unexpectedly, we identified two distinct types of prolate head structures, exhibiting a 36-degree relative rotation in the top cap region. Additionally, the head-to-tail interface demonstrated flexibility. Our structures provide the first high-resolution cryo-EM data of a mycobacteriophage with a prolate head, as well as detailed structural information of the head-to-tail interface and head-proximal tail region in this phage group. These findings advance our understanding of assembly mechanisms in tailed bacteriophages.

Cryo-EM reveals structural diversity in prolate-headed mycobacteriophage Mycofy1.,Li X, Shao Q, Li L, Xie L, Ruan Z, Fang Q J Mol Biol. 2025 Apr 3:169126. doi: 10.1016/j.jmb.2025.169126. PMID:40187685<ref>PMID:40187685</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9lw8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycolicibacterium phage Mycofy1]]
[[Category: Fang Q]]
[[Category: Li L]]
[[Category: Li X]]
[[Category: Ruan Z]]
[[Category: Shao Q]]
[[Category: Xie L]]