View source for 9meb


==Photoactivation in Bacteriophytochrome, 100 ps structure==
<StructureSection load='9meb' size='340' side='right'caption='[[9meb]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9meb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Stigmatella_aurantiaca Stigmatella aurantiaca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9MEB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9MEB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9meb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9meb OCA], [https://pdbe.org/9meb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9meb RCSB], [https://www.ebi.ac.uk/pdbsum/9meb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9meb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q09E27_STIAD Q09E27_STIAD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Myxobacteria are non-photosynthetic, soil-dwelling bacteria distinguished by a multicellular stage in their life cycle known as fruiting bodies that are stimulated by light. Myxobacterial phytochromes are candidates for the perception of red-light. The mechanism how light is perceived and converted to a physiological response is unknown. Here, time-resolved serial femtosecond crystallographic (TR-SFX) experiments were conducted on microcrystals of the photosensory core module of the Stigmatella aurantiaca bacteriophytochrome 2 (SaBphP2). Initial events of the Z to E isomerization reaction of the covalently bound, open-chain tetrapyrrole biliverdin (BV) chromophore were determined. At 3 ps after light activation, the BV ring-D assumes a configuration needed for the isomerization. At 100 ps, a mixture of BV in the Z or E configuration is observed in subunit A, while in the other subunit the chromophore remains in the Z configuration. In conjunction with prior results, these structures reveal the molecular mechanism of phytochrome activation in the photomorphogenesis of the myxobacteria and provide the molecular foundation for physiological responses to red light in other bacteria.

Observation of early events in the photoactivation of Myxobacterial phytochrome using time-resolved serial femtosecond crystallography.,Malla TN, Aldama L, Leon V, Feliz D, Hu H, Thomas I, Cellini A, Wahlgren WY, Nimmrich A, Botha S, Sierra R, Hunter MS, Poitevin F, Lisova S, Batyuk A, Gate G, Jernigan R, Kupitz CJ, Maj P, Meszaros P, Kurttila M, Monrroy L, Luo F, Owada S, Kang J, Slavov C, Maj M, Gautier C, Kashipathy M, Tolstikova A, Mariani V, Barty A, Moss F, Schwander P, Liu H, Boutet S, Fromme P, Takala H, Ihalainen JA, Weierstall U, Westenhoff S, Stojkovic EA, Schmidt M Commun Chem. 2025 Jun 12;8(1):183. doi: 10.1038/s42004-025-01578-z. PMID:40506475<ref>PMID:40506475</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9meb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Stigmatella aurantiaca]]
[[Category: Malla T]]
[[Category: Schmidt M]]