View source for 9mkp


==CAEV CA Pentamer Assembled via Liposome Templating==
<StructureSection load='9mkp' size='340' side='right'caption='[[9mkp]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9mkp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caprine_arthritis_encephalitis_virus_strain_Cork Caprine arthritis encephalitis virus strain Cork]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9MKP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9MKP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9mkp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9mkp OCA], [https://pdbe.org/9mkp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9mkp RCSB], [https://www.ebi.ac.uk/pdbsum/9mkp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9mkp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GAG_CAEVC GAG_CAEVC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lentiviruses require a mature capsid to package and traffic their viral genome for successful infection and propagation. Although the HIV-1 capsid structure has been extensively studied, structural information is lacking for other lentiviral capsids, limiting our understanding. Using cryo-electron microscopy (cryo-EM) and a liposome-templating system, we assembled capsid-like particles (CLPs) and resolved capsid protein (CA) pentamer and hexamer lattice structures from the two major phylogenetic groups of small ruminant lentiviruses (SRLVs). These structures exhibit an overall lattice organization like HIV-1 but differ in key characteristics, notably the absence of inositol hexakisphosphate (IP6) in the SRLV CA lattice horizontal line a critical factor for HIV-1 capsid assembly and function. Additionally, SRLV CA pentamers show a unique N-terminal domain orientation, providing insights into SRLV capsid assembly mechanisms. These observations, together with our molecular dynamics (MD) simulation, results suggest a possible mechanism for importing deoxynucleotide triphosphate (dNTP) molecules into SRLV capsids. Furthermore, key regions of host factor interaction, such as the CypA binding motifs, have diverged in the SRLV CA assemblies. Our results contribute to understanding the SRLV lentiviral capsids which may facilitate structure-based inhibitor design strategies.

Exploring the Structural Divergence of HIV and SRLV Lentiviral Capsids.,Arizaga F Jr, Freniere C, Rey JS, Cook M, Wu C, Perilla JR, Xiong Y J Am Chem Soc. 2025 Sep 10;147(36):32883-32895. doi: 10.1021/jacs.5c09436. Epub , 2025 Aug 29. PMID:40878534<ref>PMID:40878534</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9mkp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Caprine arthritis encephalitis virus strain Cork]]
[[Category: Large Structures]]
[[Category: Arizaga F]]
[[Category: Freniere C]]
[[Category: Xiong Y]]