View source for 9mmw


==CRISPR-associated deaminase Cad1 in cA4 bound in hexamer form refined against the consensus map==
<StructureSection load='9mmw' size='340' side='right'caption='[[9mmw]], [[Resolution|resolution]] 2.36&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9mmw]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoanaerobaculum_aquaticum Thermoanaerobaculum aquaticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9MMW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9MMW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.36&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A23:ADENOSINE-5-PHOSPHATE-2,3-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9mmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9mmw OCA], [https://pdbe.org/9mmw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9mmw RCSB], [https://www.ebi.ac.uk/pdbsum/9mmw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9mmw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A062XY19_9BACT A0A062XY19_9BACT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The recently identified CARF (CRISPR-associated Rossman-fold) family of proteins play a critical role in prokaryotic defense, mediating cOA (cyclic oligoadenylate)-stimulated ancillary immune responses in the type III CRISPR-Cas systems. Whereas most previously characterized CARF proteins contain nucleic acids or protein degradation effectors, a subset of the family, including the CARF-fused adenosine deaminase (ADA) (Cad1), has recently been shown to convert ATP to ITP. The enzymatic mechanism and the activation process of Cad1, however, remain incompletely understood. Here we present biochemical and structural analyses of a ring nuclease Cad1, revealing its substrate binding specificity and a sequential activation process by cOAs. Despite an overall structural similarity to canonical ADA enzymes, the ADA domain of Cad1 possesses unique structural features that confer a specificity for ATP. Supported by mutational analysis, our structural work demonstrates an allosteric link between the cOA-binding CARF and the ADA domain through a protein network within the hexameric enzyme assembly. Binding of a cA4 molecule to paired CARF domains induces a twisting of the linked ADA domains around one another, which remodels their active sites and alters interactions with neighboring ADA domains, thereby driving a sequential conformational activation mechanism.

The twist-and-squeeze activation of CARF-fused adenosine deaminase by cyclic oligoadenylates.,Whyms C, Zhao Y, Addo-Yobo D, He H, Whittington AC, Trasanidou D, Salazar CRP, Staals RHJ, Li H EMBO J. 2025 Oct 17. doi: 10.1038/s44318-025-00578-y. PMID:41107544<ref>PMID:41107544</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9mmw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermoanaerobaculum aquaticum]]
[[Category: Li H]]
[[Category: Whyms C]]
[[Category: Zhao Y]]