View source for 9muf


==Cryo-EM structure of the IFI16-PYD filament==
<StructureSection load='9muf' size='340' side='right'caption='[[9muf]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9muf]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9MUF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9MUF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9muf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9muf OCA], [https://pdbe.org/9muf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9muf RCSB], [https://www.ebi.ac.uk/pdbsum/9muf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9muf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0AA49X8J6_HUMAN A0AA49X8J6_HUMAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In response to various intracellular stress or damage-associated signals, inflammasomes can be activated and trigger a pyroptotic cell death process through the sequential assembly of structurally compatible and interacting filamentous oligomers involving the pyrin domains (PYD) of important inflammasome components. The PYD-containing interferon-inducible protein 16 (IFI16) has been suggested as a viral DNA sensor that can induce inflammasome formation, but it also has other inflammasome-independent functions, including interferon production. Here, the cryo-EM structure of the filament assembled by the PYD of human IFI16 reveals a helical architecture distinct from inflammasome PYD filaments. In silico interface energy calculations suggest that the helical architecture of the IFI16(PYD) filament prevents interactions with inflammasome PYD filaments. Biochemical and cell biology experiments consistently demonstrate that IFI16 does not directly interact with inflammasome pyrin domains. Together, our results provide insights into the structural basis of the inflammasome-independent functions of IFI16, and also show that strict architectural compatibility requirements for interactions contribute to the signal transduction specificity in inflammasome signaling.

Structural insights into the atypical filament assembly of pyrin domain-containing IFI16.,Garg A, Niedzialkowska E, Zhou JJ, Moh J, Egelman EH, Sohn J EMBO J. 2025 Nov 5. doi: 10.1038/s44318-025-00626-7. PMID:41193640<ref>PMID:41193640</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9muf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Egelman EH]]
[[Category: Garg A]]
[[Category: Niedzialkowska E]]
[[Category: Sohn J]]