View source for 9n15


==Cryo-EM structure of HCoV-HKU1 glycoprotein D1 Domain in complex with 9OAc-GD3(mutant T31VPR34 to GGGG)==
<StructureSection load='9n15' size='340' side='right'caption='[[9n15]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9n15]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_coronavirus_HKU1 Human coronavirus HKU1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9N15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9N15 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1AR1:(2~{R},4~{S},5~{R},6~{R})-5-acetamido-6-[(1~{S},2~{R})-2-[(2~{S},4~{S},5~{R},6~{R})-5-acetamido-6-[(1~{R},2~{R})-3-acetyloxy-1,2-bis(oxidanyl)propyl]-2-carboxy-4-oxidanyl-oxan-2-yl]oxy-1,3-bis(oxidanyl)propyl]-2,4-bis(oxidanyl)oxane-2-carboxylic+acid'>A1AR1</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9n15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9n15 OCA], [https://pdbe.org/9n15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9n15 RCSB], [https://www.ebi.ac.uk/pdbsum/9n15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9n15 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPIKE_CVHN1 SPIKE_CVHN1] S1 attaches the virion to the cell membrane by interacting with cell receptors, initiating the infection.  S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Presumably interacts with target cell lipid raft after cell attachment (By similarity).
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</StructureSection>
[[Category: Human coronavirus HKU1]]
[[Category: Large Structures]]
[[Category: Jin M]]
[[Category: Rini JM]]