View source for 9nk8


==Thermothelomyces thermophilus SAM complex bound to darobactin A==
<StructureSection load='9nk8' size='340' side='right'caption='[[9nk8]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9nk8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_khanii Photorhabdus khanii] and [https://en.wikipedia.org/wiki/Thermothelomyces_thermophilus Thermothelomyces thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9NK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9NK8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ERG:ERGOSTEROL'>ERG</scene>, <scene name='pdbligand=UX8:(2~{S},3~{R})-2-azanyl-3-(1~{H}-indol-3-yl)-3-oxidanyl-propanoic+acid'>UX8</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9nk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9nk8 OCA], [https://pdbe.org/9nk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9nk8 RCSB], [https://www.ebi.ac.uk/pdbsum/9nk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9nk8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G2QAT9_THET4 G2QAT9_THET4] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The folding and insertion of beta-barrel proteins into the mitochondrial outer membrane is facilitated by the sorting and assembly machinery (SAM) complex. Here we report two 2.8 A cryo-EM structures of the Thermothelomyces thermophilus SAM complex in the absence of substrate in which the Sam50 lateral gate adopts two different conformations: the first is a closed lateral gate as observed in previously published structures, while the second contains a Sam50 with the first four beta-strands rotated outwards by approximately 45 degrees , resulting in an open lateral gate. The observed monomeric open conformation contrasts our previous work where the open conformation was adopted by non-physiological up-down dimers. To understand how these lateral gate dynamics are influenced by substrate, we studied the interaction of the SAM complex with a beta-signal peptide mimic, darobactin A. Darobactin A binds to the SAM complex with nanomolar affinity and inhibits the import and assembly of mitochondrial beta-barrel proteins in vitro. Lastly, we solved a 3.0 A cryo-EM structure of the Thermothelomyces thermophilus SAM complex bound to darobactin A, which reveals that darobactin A stabilizes the Sam50 lateral gate similar to the open conformation by binding to strand beta1, therefore blocking beta-barrel biogenesis.

The dynamic lateral gate of the mitochondrial beta-barrel biogenesis machinery is blocked by darobactin A.,Diederichs KA, Botos I, Hayashi S, Gutishvili G, Kotov V, Kuo K, Iinishi A, Cooper G, Schwarz B, Celia H, Marlovits TC, Lewis K, Gumbart JC, Mindell JA, Buchanan SK Nat Commun. 2025 Nov 20. doi: 10.1038/s41467-025-66417-0. PMID:41266328<ref>PMID:41266328</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 9nk8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Photorhabdus khanii]]
[[Category: Thermothelomyces thermophilus]]
[[Category: Botos I]]
[[Category: Buchanan SK]]
[[Category: Diederichs K]]